Obesity modulates the expression of haptoglobin in the white adipose tissue via TNFα

C. Chiellini, A. Bertacca, S. E. Novelli, C. Z. Görgün, A. Ciccarone, A. Giordano, H. Xu, A. Soukas, M. Costa, D. Gandini, R. Dimitri, P. Bottone, P. Cecchetti, E. Pardini, L. Perego, R. Navalesi, F. Folli, L. Benzi, S. Cinti, J. M. FriedmanG. S. Hotamisligil, M. Maffei

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Increase in adipose mass results in obesity and modulation of several factors in white adipose tissue (WAT). Two important examples are tumor necrosis factor α (TNFα) and leptin, both of which are upregulated in adipose tissue in obesity. In order to isolate genes differentially expressed in the WAT of genetically obese db/db mice compared to their lean littermates, we performed RNA fingerprinting and identified haptoglobin (Hp), which is significantly upregulated in the obese animals. Hp is a glycoprotein induced by a number of cytokines, LPS (Lipopolysaccharide), and more generally by inflammation. A significant upregulation of WAT Hp expression was also evident in several experimental obese models including the yellow agouti (/) Ay, ob/ob and goldthioglucose-treated mice (10-, 8-, and 7-fold, respectively). To identify the potential signals for an increase in Hp expression in obesity, we examined leptin and TNFα in vivo. Wild type animals treated with recombinant leptin did not show any alteration in WAT Hp expression compared to controls that were food restricted to the level of intake of the treated animals. On the other hand, Hp expression was induced in mice transgenically expressing TNFα in adipose tissue. Finally, a significant down-regulation of WAT Hp mRNA was observed in ob/ob mice deficient in TNFα function, when compared to the ob/ob controls. These results demonstrate that haptoglobin expression in WAT is increased in obesity in rodents and TNFα is an important signal for this regulation.

Original languageEnglish (US)
Pages (from-to)251-258
Number of pages8
JournalJournal of Cellular Physiology
Volume190
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

White Adipose Tissue
Haptoglobins
Tumor Necrosis Factor-alpha
Obesity
Tissue
Leptin
Animals
Adipose Tissue
Wild Animals
Lipopolysaccharides
Rodentia
Glycoproteins
Theoretical Models
Up-Regulation
Down-Regulation
Genes
Modulation
RNA
Cytokines
Inflammation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Chiellini, C., Bertacca, A., Novelli, S. E., Görgün, C. Z., Ciccarone, A., Giordano, A., ... Maffei, M. (2002). Obesity modulates the expression of haptoglobin in the white adipose tissue via TNFα. Journal of Cellular Physiology, 190(2), 251-258. https://doi.org/10.1002/jcp.10061

Obesity modulates the expression of haptoglobin in the white adipose tissue via TNFα. / Chiellini, C.; Bertacca, A.; Novelli, S. E.; Görgün, C. Z.; Ciccarone, A.; Giordano, A.; Xu, H.; Soukas, A.; Costa, M.; Gandini, D.; Dimitri, R.; Bottone, P.; Cecchetti, P.; Pardini, E.; Perego, L.; Navalesi, R.; Folli, F.; Benzi, L.; Cinti, S.; Friedman, J. M.; Hotamisligil, G. S.; Maffei, M.

In: Journal of Cellular Physiology, Vol. 190, No. 2, 2002, p. 251-258.

Research output: Contribution to journalArticle

Chiellini, C, Bertacca, A, Novelli, SE, Görgün, CZ, Ciccarone, A, Giordano, A, Xu, H, Soukas, A, Costa, M, Gandini, D, Dimitri, R, Bottone, P, Cecchetti, P, Pardini, E, Perego, L, Navalesi, R, Folli, F, Benzi, L, Cinti, S, Friedman, JM, Hotamisligil, GS & Maffei, M 2002, 'Obesity modulates the expression of haptoglobin in the white adipose tissue via TNFα', Journal of Cellular Physiology, vol. 190, no. 2, pp. 251-258. https://doi.org/10.1002/jcp.10061
Chiellini C, Bertacca A, Novelli SE, Görgün CZ, Ciccarone A, Giordano A et al. Obesity modulates the expression of haptoglobin in the white adipose tissue via TNFα. Journal of Cellular Physiology. 2002;190(2):251-258. https://doi.org/10.1002/jcp.10061
Chiellini, C. ; Bertacca, A. ; Novelli, S. E. ; Görgün, C. Z. ; Ciccarone, A. ; Giordano, A. ; Xu, H. ; Soukas, A. ; Costa, M. ; Gandini, D. ; Dimitri, R. ; Bottone, P. ; Cecchetti, P. ; Pardini, E. ; Perego, L. ; Navalesi, R. ; Folli, F. ; Benzi, L. ; Cinti, S. ; Friedman, J. M. ; Hotamisligil, G. S. ; Maffei, M. / Obesity modulates the expression of haptoglobin in the white adipose tissue via TNFα. In: Journal of Cellular Physiology. 2002 ; Vol. 190, No. 2. pp. 251-258.
@article{4e612167f8ed4b6ba0c98b51c2d00bca,
title = "Obesity modulates the expression of haptoglobin in the white adipose tissue via TNFα",
abstract = "Increase in adipose mass results in obesity and modulation of several factors in white adipose tissue (WAT). Two important examples are tumor necrosis factor α (TNFα) and leptin, both of which are upregulated in adipose tissue in obesity. In order to isolate genes differentially expressed in the WAT of genetically obese db/db mice compared to their lean littermates, we performed RNA fingerprinting and identified haptoglobin (Hp), which is significantly upregulated in the obese animals. Hp is a glycoprotein induced by a number of cytokines, LPS (Lipopolysaccharide), and more generally by inflammation. A significant upregulation of WAT Hp expression was also evident in several experimental obese models including the yellow agouti (/) Ay, ob/ob and goldthioglucose-treated mice (10-, 8-, and 7-fold, respectively). To identify the potential signals for an increase in Hp expression in obesity, we examined leptin and TNFα in vivo. Wild type animals treated with recombinant leptin did not show any alteration in WAT Hp expression compared to controls that were food restricted to the level of intake of the treated animals. On the other hand, Hp expression was induced in mice transgenically expressing TNFα in adipose tissue. Finally, a significant down-regulation of WAT Hp mRNA was observed in ob/ob mice deficient in TNFα function, when compared to the ob/ob controls. These results demonstrate that haptoglobin expression in WAT is increased in obesity in rodents and TNFα is an important signal for this regulation.",
author = "C. Chiellini and A. Bertacca and Novelli, {S. E.} and G{\"o}rg{\"u}n, {C. Z.} and A. Ciccarone and A. Giordano and H. Xu and A. Soukas and M. Costa and D. Gandini and R. Dimitri and P. Bottone and P. Cecchetti and E. Pardini and L. Perego and R. Navalesi and F. Folli and L. Benzi and S. Cinti and Friedman, {J. M.} and Hotamisligil, {G. S.} and M. Maffei",
year = "2002",
doi = "10.1002/jcp.10061",
language = "English (US)",
volume = "190",
pages = "251--258",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Obesity modulates the expression of haptoglobin in the white adipose tissue via TNFα

AU - Chiellini, C.

AU - Bertacca, A.

AU - Novelli, S. E.

AU - Görgün, C. Z.

AU - Ciccarone, A.

AU - Giordano, A.

AU - Xu, H.

AU - Soukas, A.

AU - Costa, M.

AU - Gandini, D.

AU - Dimitri, R.

AU - Bottone, P.

AU - Cecchetti, P.

AU - Pardini, E.

AU - Perego, L.

AU - Navalesi, R.

AU - Folli, F.

AU - Benzi, L.

AU - Cinti, S.

AU - Friedman, J. M.

AU - Hotamisligil, G. S.

AU - Maffei, M.

PY - 2002

Y1 - 2002

N2 - Increase in adipose mass results in obesity and modulation of several factors in white adipose tissue (WAT). Two important examples are tumor necrosis factor α (TNFα) and leptin, both of which are upregulated in adipose tissue in obesity. In order to isolate genes differentially expressed in the WAT of genetically obese db/db mice compared to their lean littermates, we performed RNA fingerprinting and identified haptoglobin (Hp), which is significantly upregulated in the obese animals. Hp is a glycoprotein induced by a number of cytokines, LPS (Lipopolysaccharide), and more generally by inflammation. A significant upregulation of WAT Hp expression was also evident in several experimental obese models including the yellow agouti (/) Ay, ob/ob and goldthioglucose-treated mice (10-, 8-, and 7-fold, respectively). To identify the potential signals for an increase in Hp expression in obesity, we examined leptin and TNFα in vivo. Wild type animals treated with recombinant leptin did not show any alteration in WAT Hp expression compared to controls that were food restricted to the level of intake of the treated animals. On the other hand, Hp expression was induced in mice transgenically expressing TNFα in adipose tissue. Finally, a significant down-regulation of WAT Hp mRNA was observed in ob/ob mice deficient in TNFα function, when compared to the ob/ob controls. These results demonstrate that haptoglobin expression in WAT is increased in obesity in rodents and TNFα is an important signal for this regulation.

AB - Increase in adipose mass results in obesity and modulation of several factors in white adipose tissue (WAT). Two important examples are tumor necrosis factor α (TNFα) and leptin, both of which are upregulated in adipose tissue in obesity. In order to isolate genes differentially expressed in the WAT of genetically obese db/db mice compared to their lean littermates, we performed RNA fingerprinting and identified haptoglobin (Hp), which is significantly upregulated in the obese animals. Hp is a glycoprotein induced by a number of cytokines, LPS (Lipopolysaccharide), and more generally by inflammation. A significant upregulation of WAT Hp expression was also evident in several experimental obese models including the yellow agouti (/) Ay, ob/ob and goldthioglucose-treated mice (10-, 8-, and 7-fold, respectively). To identify the potential signals for an increase in Hp expression in obesity, we examined leptin and TNFα in vivo. Wild type animals treated with recombinant leptin did not show any alteration in WAT Hp expression compared to controls that were food restricted to the level of intake of the treated animals. On the other hand, Hp expression was induced in mice transgenically expressing TNFα in adipose tissue. Finally, a significant down-regulation of WAT Hp mRNA was observed in ob/ob mice deficient in TNFα function, when compared to the ob/ob controls. These results demonstrate that haptoglobin expression in WAT is increased in obesity in rodents and TNFα is an important signal for this regulation.

UR - http://www.scopus.com/inward/record.url?scp=0036136567&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036136567&partnerID=8YFLogxK

U2 - 10.1002/jcp.10061

DO - 10.1002/jcp.10061

M3 - Article

C2 - 11807829

AN - SCOPUS:0036136567

VL - 190

SP - 251

EP - 258

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 2

ER -