Obesity-associated alterations in cardiac connexin-43 and PKC signaling are attenuated by melatonin and omega-3 fatty acids in female rats

Tamara Egan Benova, Csilla Viczenczova, Barbara Szeiffova Bacova, Vladimir Knezl, Victor Dosenko, Hana Rauchova, Michal Zeman, Russel J Reiter, Narcis Tribulova

Research output: Contribution to journalArticle

Abstract

We aimed to explore whether specific high-sucrose intake in older female rats affects myocardial electrical coupling protein, connexin-43 (Cx43), protein kinase C (PKC) signaling, miR-1 and miR-30a expression, and susceptibility of the heart to malignant arrhythmias. Possible benefit of the supplementation with melatonin (40 µg/ml/day) and omega-3 polyunsaturated fatty acids (Omacor, 25 g/kg of rat chow) was examined as well. Results have shown that 8 weeks lasting intake of 30% sucrose solution increased serum cholesterol, triglycerides, body weight, heart weight, and retroperitoneal adipose tissues. It was accompanied by downregulation of cardiac Cx43 and PKCε signaling along with an upregulation of myocardial PKCδ and miR-30a rendering the heart prone to ventricular arrhythmias. There was a clear benefit of melatonin or omega-3 PUFA supplementation due to their antiarrhythmic effects associated with the attenuation of myocardial Cx43, PKC, and miR-30a abnormalities as well as adiposity. The potential impact of these findings may be considerable, and suggests that high-sucrose intake impairs myocardial signaling mediated by Cx43 and PKC contributing to increased susceptibility of the older obese female rat hearts to malignant arrhythmias.

Original languageEnglish (US)
JournalMolecular and Cellular Biochemistry
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Connexin 43
Omega-3 Fatty Acids
Melatonin
Protein Kinase C
Rats
Obesity
Sucrose
Cardiac Arrhythmias
Intra-Abdominal Fat
Adiposity
Unsaturated Fatty Acids
Triglycerides
Up-Regulation
Down-Regulation
Cholesterol
Body Weight
Tissue
Weights and Measures
Serum
Proteins

Keywords

  • Adiposity
  • Cardiac connexin-43
  • Female rats
  • Melatonin
  • Omega-3 PUFA
  • Sucrose diet
  • Ventricular fibrillation

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Obesity-associated alterations in cardiac connexin-43 and PKC signaling are attenuated by melatonin and omega-3 fatty acids in female rats. / Egan Benova, Tamara; Viczenczova, Csilla; Szeiffova Bacova, Barbara; Knezl, Vladimir; Dosenko, Victor; Rauchova, Hana; Zeman, Michal; Reiter, Russel J; Tribulova, Narcis.

In: Molecular and Cellular Biochemistry, 01.01.2018.

Research output: Contribution to journalArticle

Egan Benova, Tamara ; Viczenczova, Csilla ; Szeiffova Bacova, Barbara ; Knezl, Vladimir ; Dosenko, Victor ; Rauchova, Hana ; Zeman, Michal ; Reiter, Russel J ; Tribulova, Narcis. / Obesity-associated alterations in cardiac connexin-43 and PKC signaling are attenuated by melatonin and omega-3 fatty acids in female rats. In: Molecular and Cellular Biochemistry. 2018.
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abstract = "We aimed to explore whether specific high-sucrose intake in older female rats affects myocardial electrical coupling protein, connexin-43 (Cx43), protein kinase C (PKC) signaling, miR-1 and miR-30a expression, and susceptibility of the heart to malignant arrhythmias. Possible benefit of the supplementation with melatonin (40 µg/ml/day) and omega-3 polyunsaturated fatty acids (Omacor, 25 g/kg of rat chow) was examined as well. Results have shown that 8 weeks lasting intake of 30{\%} sucrose solution increased serum cholesterol, triglycerides, body weight, heart weight, and retroperitoneal adipose tissues. It was accompanied by downregulation of cardiac Cx43 and PKCε signaling along with an upregulation of myocardial PKCδ and miR-30a rendering the heart prone to ventricular arrhythmias. There was a clear benefit of melatonin or omega-3 PUFA supplementation due to their antiarrhythmic effects associated with the attenuation of myocardial Cx43, PKC, and miR-30a abnormalities as well as adiposity. The potential impact of these findings may be considerable, and suggests that high-sucrose intake impairs myocardial signaling mediated by Cx43 and PKC contributing to increased susceptibility of the older obese female rat hearts to malignant arrhythmias.",
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AU - Szeiffova Bacova, Barbara

AU - Knezl, Vladimir

AU - Dosenko, Victor

AU - Rauchova, Hana

AU - Zeman, Michal

AU - Reiter, Russel J

AU - Tribulova, Narcis

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