Nucleotide excision repair of actively transcribed versus nontranscribed DNA in rat hepatocytes: Effect of age and dietary restriction

Zhong Mao Guo, Ahmad Heydari, Arlan Richardson

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

The ability of primary cultures of rat hepatocytes to remove cyclobutane pyrimidine dimers (CPDs) from DNA fragments containing the transcriptionally active albumin gene and the transcriptionally inactive embryonic myosin heavy chain (MHC(emb)) and H-ras fragments as well as the genome overall was measured. At all UV doses studied, more CPDs were observed in the three DNA fragments and the genome overall in hepatocytes isolated from old (24-month- old) rats fed ad libitum than in young (6-month-old) rats fed ad libitum or old rats fed a calorie-restricted diet. The cultured hepatocytes preferentially removed CPDs from the albumin fragment compared to the genome overall or the MHC(emb) and H-ras fragments. The rate of repair (12 h after UV irradiation) of the albumin fragment was approximately 40% less in hepatocytes isolated from old rats than from young rats; this was due to a decrease in repair of the transcribed strand of this fragment, and dietary restriction prevented this decrease. The extent of repair (24 h after UV irradiation) of the MHC(emb) and H-ras fragments as well as the genome overall was reduced approximately 40% with age, and this decrease was reversed by dietary restriction.

Original languageEnglish (US)
Pages (from-to)228-238
Number of pages11
JournalExperimental Cell Research
Volume245
Issue number1
DOIs
StatePublished - Nov 25 1998

Keywords

  • Aging
  • DNA repair
  • Dietary restriction
  • Hepatocytes
  • Rat

ASJC Scopus subject areas

  • Cell Biology

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