Nucleosome positioning by the winged helix transcription factor HNF3

Eun Yong Shim, Christopher Woodcock, Kenneth S. Zaret

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

Nucleosome positioning at genetic regulatory sequences is not well understood. The transcriptional enhancer of the mouse serum albumin gene is active in liver, where regulatory factors occupy their target sites on three nucleosome-like particles designated N1, N2, and N3. The winged helix transcription factor HNF3 binds to two sites near the center of the N1 particle. We created dinucleosome templates using the albumin enhancer sequence and found that site-specific binding of HNF3 protein resulted in nucleosome positioning in vitro similar to that seen in liver nuclei. Thus, binding of a transcription factor can position an underlying nucleosome core.

Original languageEnglish (US)
Pages (from-to)5-10
Number of pages6
JournalGenes and Development
Volume12
Issue number1
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Keywords

  • Chromatin
  • Enhancer
  • HNF3
  • Nucleosome
  • Winged helix factor

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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