Nucleocytoplasmic release of repetitive DNA transcripts in carcinogenesis correlates with a 60 kilodalton cytoplasmic protein

Bernard T. French, Hanausek Walaszek Margaret, Zbigniew Walaszek, Dorothy E. Schumm, Thomas E. Webb

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Treatment of rats with the hepatocarcinogen 3′-methyl-4-dimethylaminoazobenzene (3′-MeDAB) causes the appearance in the liver cytosol of a 60 kilodalton oncofetal protein. The appearance of this factor occurs within 40 h of treatment and coincides with the increase in the amount of rapidly labeled RNA released from nuclei in a reconstituted cell-free system. Cross-over experiments show that this increase is due to an enhanced transport capacity of the cytosol. The 60 kilodalton RNA transport factor is also present in the cytosol of tumor cells. Addition of the 60 kilodalton factor to normal liver cytosol causes the transport of repetitive RNA sequences similar to those transported from liver nuclei to tumor cell cytosol and those transported to the tumor cell cytoplasm in vivo. This factor modifies nuclear RNA restriction, at least in part, by eliciting the transport of repetitive RNA normally retained within the nucleus of the normal cell.

Original languageEnglish (US)
Pages (from-to)45-52
Number of pages8
JournalCancer Letters
Volume23
Issue number1
DOIs
StatePublished - May 1984
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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