Nuclear cholesterol content and nucleoside triphosphatase activity are altered in the JCR:LA-cp corpulent rat

Michael P. Czubryt, James C. Russell, John Sarantopoulos, Grant N. Pierce

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A nuclear pore complex-associated nucleoside triphosphatase (NTPase) activity is believed to provide energy for nuclear export of poly(A)+ mRNA. This study was initiated to determine if nuclear membrane lipid composition is altered during chronic hyperlipidemia, and what effect this has on NTPase activity. The JCR:LA-cp corpulent rat model is characterized by severe hypertriglyceridemia and moderate hypercholesterolemia, and thus represents an ideal animal model in which to study nuclear cholesterol and NTPase activity. NTPase activity was markedly increased in purified hepatic nuclei from corpulent female JCR:LA-cp rats in comparison to lean control rats as a function of assay time, [GTP], [ATP], and [Mg2+]. Nuclear membrane cholesterol and phospholipid content were significantly elevated in the corpulent animals. Nuclei of corpulent animals were less resistant to salt- induced lysis than nuclei of lean animals, suggesting a change in relative membrane integrity. Together, these results indicate that altered lipid metabolism in a genetic corpulent animal model can lead to changes in nuclear membrane lipid composition, which in turn may alter nuclear membrane NTPase activity and integrity.

Original languageEnglish (US)
Pages (from-to)349-357
Number of pages9
JournalJournal of Cellular Biochemistry
Volume63
Issue number3
DOIs
StatePublished - Dec 1 1996
Externally publishedYes

Keywords

  • NTPase
  • hypercholesterolemia
  • hyperlipidemia
  • nuclear membrane
  • obesity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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