NRG1, a repressor of filamentous growth in C.albicans, is down-regulated during filament induction

B. R. Braun, D. Kadosh, A. D. Johnson

Research output: Contribution to journalArticlepeer-review

275 Scopus citations


In response to a variety of external signals, the fungal pathogen Candida albicans undergoes a transition between ellipsoidal single cells (blastospores) and filaments composed of elongated cells attached end-to-end. Here we identify a DNA-binding protein, Nrg1, that represses filamentous growth in Candida probably by acting through the co-repressor Tup1. nrg1 mutant cells are predominantly filamentous under non-filament-inducing conditions and their colony morphology resembles that of tup1 mutants. We also identify two filament-specific genes, ECE1 and HWP1, whose transcription is repressed by Nrg1 under non-inducing conditions. These genes constitute a subset of those under Tup1 control, providing further evidence that Nrg1 acts by recruiting Tup1 to target genes. We show that growth in serum at 37°C, a potent inducer of filamentous growth, causes a reduction of NRG1 mRNA, suggesting that filamentous growth is induced by the down-regulation of NRG1. Consistent with this idea, expression of NRG1 from a non-regulated promoter partially blocks the induction of filamentous growth.

Original languageEnglish (US)
Pages (from-to)4753-4761
Number of pages9
JournalEMBO Journal
Issue number17
StatePublished - Sep 3 2001
Externally publishedYes


  • Candida albicans
  • Filaments
  • Hyphae
  • Nrg1
  • Tup1

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • Molecular Biology
  • General Neuroscience


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