Novel truncated isoforms of constitutive serum amyloid A detected by MALDI mass spectrometry

Zachlyn N. Farwig; Catherine J. McNeal; Danny Little; Clinton E. Baisden; Ronald D. Macfarlane

doi:10.1016/j.bbrc.2005.04.129

Novel truncated isoforms of constitutive serum amyloid A detected by MALDI mass spectrometry

Zachlyn N. Farwig, Catherine J. McNeal, Danny Little, Clinton E. Baisden, Ronald D. Macfarlane

Department of Cardiothoracic Surgery

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The main focus of the serum amyloid A (SAA) family has been on the acute phase isoforms. However, the constitutive isoform (SAA4) may have a strong effect on the metabolism of human serum lipoproteins. In this study, the SAA4 protein was examined in the high-density lipoprotein fraction of both healthy and diseased individuals. Novel isoforms of SAA4 were detected using ultracentrifugation combined with solid-phase extraction and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Three truncated isoforms were identified as well as two glycosylated isoforms. Patterns of isoform distribution may be significant for assessment of cardiovascular risk as well as direction of patient treatment.

Original language	English (US)
Pages (from-to)	352-356
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	332
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2005.04.129
State	Published - Jul 1 2005

Keywords

HDL
MALDI-TOF MS
SAA

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.04.129

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title = "Novel truncated isoforms of constitutive serum amyloid A detected by MALDI mass spectrometry",

abstract = "The main focus of the serum amyloid A (SAA) family has been on the acute phase isoforms. However, the constitutive isoform (SAA4) may have a strong effect on the metabolism of human serum lipoproteins. In this study, the SAA4 protein was examined in the high-density lipoprotein fraction of both healthy and diseased individuals. Novel isoforms of SAA4 were detected using ultracentrifugation combined with solid-phase extraction and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Three truncated isoforms were identified as well as two glycosylated isoforms. Patterns of isoform distribution may be significant for assessment of cardiovascular risk as well as direction of patient treatment.",

keywords = "HDL, MALDI-TOF MS, SAA",

author = "Farwig, {Zachlyn N.} and McNeal, {Catherine J.} and Danny Little and Baisden, {Clinton E.} and Macfarlane, {Ronald D.}",

note = "Funding Information: This work was supported by the NIH, Heart and Lung Institute (HL 68794, HL 54566), Scott, White, Sherwood and Brindley Foundation (projects 1532 and 1791) and the Welch Foundation (A-0258). Use of the TAMU/LBMS-Applications Laboratory and Dr. Shane Tichy is acknowledged. We also thank Judy Herrick, Gabriela Gonzales, Erlene Fritz, and Christopher Herrick for their work in providing clinical samples from Scott & White Hospital, Temple, TX. ",

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doi = "10.1016/j.bbrc.2005.04.129",

language = "English (US)",

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pages = "352--356",

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T1 - Novel truncated isoforms of constitutive serum amyloid A detected by MALDI mass spectrometry

AU - Farwig, Zachlyn N.

AU - McNeal, Catherine J.

AU - Little, Danny

AU - Baisden, Clinton E.

AU - Macfarlane, Ronald D.

N1 - Funding Information: This work was supported by the NIH, Heart and Lung Institute (HL 68794, HL 54566), Scott, White, Sherwood and Brindley Foundation (projects 1532 and 1791) and the Welch Foundation (A-0258). Use of the TAMU/LBMS-Applications Laboratory and Dr. Shane Tichy is acknowledged. We also thank Judy Herrick, Gabriela Gonzales, Erlene Fritz, and Christopher Herrick for their work in providing clinical samples from Scott & White Hospital, Temple, TX.

PY - 2005/7/1

Y1 - 2005/7/1

N2 - The main focus of the serum amyloid A (SAA) family has been on the acute phase isoforms. However, the constitutive isoform (SAA4) may have a strong effect on the metabolism of human serum lipoproteins. In this study, the SAA4 protein was examined in the high-density lipoprotein fraction of both healthy and diseased individuals. Novel isoforms of SAA4 were detected using ultracentrifugation combined with solid-phase extraction and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Three truncated isoforms were identified as well as two glycosylated isoforms. Patterns of isoform distribution may be significant for assessment of cardiovascular risk as well as direction of patient treatment.

AB - The main focus of the serum amyloid A (SAA) family has been on the acute phase isoforms. However, the constitutive isoform (SAA4) may have a strong effect on the metabolism of human serum lipoproteins. In this study, the SAA4 protein was examined in the high-density lipoprotein fraction of both healthy and diseased individuals. Novel isoforms of SAA4 were detected using ultracentrifugation combined with solid-phase extraction and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Three truncated isoforms were identified as well as two glycosylated isoforms. Patterns of isoform distribution may be significant for assessment of cardiovascular risk as well as direction of patient treatment.

KW - HDL

KW - MALDI-TOF MS

KW - SAA

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AN - SCOPUS:19444367338

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JO - Biochemical and Biophysical Research Communications

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ER -

Novel truncated isoforms of constitutive serum amyloid A detected by MALDI mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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