TY - JOUR
T1 - Novel strategies for enhancing shotgun lipidomics for comprehensive analysis of cellular lipidomes
AU - Hu, Changfeng
AU - Wang, Chunyan
AU - He, Lijiao
AU - Han, Xianlin
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/11
Y1 - 2019/11
N2 - Shotgun lipidomics is one of the most powerful tools in analysis of cellular lipidomes in lipidomics, which directly analyzes lipids from lipid extracts of diverse biological samples with high accuracy/precision. However, despite its great advances in high throughput analysis of cellular lipidomes, low coverage of poorly ionized lipids, especially those species in very low abundance, and some types of isomers within complex lipid extracts by shotgun lipidomics remains a huge challenge. In the past few years, many strategies have been developed to enhance shotgun lipidomics for comprehensive analysis of lipid species. Chemical derivatization represents one of the most attractive and effective strategies, already receiving considerable attention. This review focuses on novel advanced derivatization strategies for enhancing shotgun lipidomics. It is anticipated that with the development of enhanced strategies, shotgun lipidomics can make greater contributions to biological and biomedical research.
AB - Shotgun lipidomics is one of the most powerful tools in analysis of cellular lipidomes in lipidomics, which directly analyzes lipids from lipid extracts of diverse biological samples with high accuracy/precision. However, despite its great advances in high throughput analysis of cellular lipidomes, low coverage of poorly ionized lipids, especially those species in very low abundance, and some types of isomers within complex lipid extracts by shotgun lipidomics remains a huge challenge. In the past few years, many strategies have been developed to enhance shotgun lipidomics for comprehensive analysis of lipid species. Chemical derivatization represents one of the most attractive and effective strategies, already receiving considerable attention. This review focuses on novel advanced derivatization strategies for enhancing shotgun lipidomics. It is anticipated that with the development of enhanced strategies, shotgun lipidomics can make greater contributions to biological and biomedical research.
KW - Bis(monoacylglycero)phosphate
KW - Chemical derivatization
KW - Multi-dimensional mass spectrometry
KW - Plasmalogen
KW - Polyphosphoinositides
KW - Shotgun lipidomics
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U2 - 10.1016/j.trac.2018.11.028
DO - 10.1016/j.trac.2018.11.028
M3 - Review article
C2 - 32647401
AN - SCOPUS:85057621379
SN - 0165-9936
VL - 120
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
M1 - 115330
ER -