Novel strategies for enhancing shotgun lipidomics for comprehensive analysis of cellular lipidomes

Changfeng Hu, Chunyan Wang, Lijiao He, Xianlin Han

Research output: Contribution to journalReview articlepeer-review

29 Scopus citations


Shotgun lipidomics is one of the most powerful tools in analysis of cellular lipidomes in lipidomics, which directly analyzes lipids from lipid extracts of diverse biological samples with high accuracy/precision. However, despite its great advances in high throughput analysis of cellular lipidomes, low coverage of poorly ionized lipids, especially those species in very low abundance, and some types of isomers within complex lipid extracts by shotgun lipidomics remains a huge challenge. In the past few years, many strategies have been developed to enhance shotgun lipidomics for comprehensive analysis of lipid species. Chemical derivatization represents one of the most attractive and effective strategies, already receiving considerable attention. This review focuses on novel advanced derivatization strategies for enhancing shotgun lipidomics. It is anticipated that with the development of enhanced strategies, shotgun lipidomics can make greater contributions to biological and biomedical research.

Original languageEnglish (US)
Article number115330
JournalTrAC - Trends in Analytical Chemistry
StatePublished - Nov 2019


  • Bis(monoacylglycero)phosphate
  • Chemical derivatization
  • Multi-dimensional mass spectrometry
  • Plasmalogen
  • Polyphosphoinositides
  • Shotgun lipidomics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy


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