Novel PLA- and PCL-HA porous 3D scaffolds prepared by robocasting facilitate MC3T3-E1 subclone 4 cellular attachment and growth

V. G. Varanasi, J. Russias, E. Saiz, P. M. Loomer, A. P. Tomsia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Novel robocast poly-caprolactone- and poly-lactic acid-hydroxyapatite composites were fabricated as planar films (2D) and multi-layered scaffolds (3D) (PCL-HA and PLA-HA, respectively). These materials contained 70 wt.% HA and were tested in vitro. Cell-free in vitro testing was conducted prior to cell culture testing to determine the effect that media conditions had on each material. Scanning electron microscopy (SEM) analysis showed that 3D scaffolds had exposed HA crystals on the rods of the mesh-like structure. Electron dispersive x-ray analysis (EDX) showed that the Ca: P ratio for HA was 1.3 and that the HA was carbonate-free. X-ray diffraction analysis (XRD) showed that the HA had a random crystallite distribution. Cell culture studies showed that both 2D films and 3D scaffolds supported osteoblast attachment and growth. Cell densities after 7 d of proliferation were higher on 2D films as compared to 3D scaffolds. Higher cell densities were observed for fully-dense HA as compared to 3D scaffolds. In general, PLA-HA scaffolds had higher cell densities than PCL-HA scaffolds. SEM analysis showed osteoblast attachment (via lamellipodia and filopodia) to the scaffolds, with cell layers wrapped around the mesh structure. Both polymer-HA composites degraded, with HA-PLA scaffolds exhibiting increased formation of micron-sized pores while PCL-HA composites exhibited increased formation of surface fiber bundles interconnected between features of the scaffold.

Original languageEnglish (US)
Title of host publicationBiomaterials Science
Subtitle of host publicationProcessing, Properties and Applications V
EditorsSusmita Bose, Amit Bandyopadhyay, Roger Narayan
PublisherAmerican Ceramic Society
Pages175-186
Number of pages12
ISBN (Electronic)9781119190028
StatePublished - Jan 1 2015
Externally publishedYes
EventNext Generation Biomaterials and Surface Properties of Biomaterials Symposia - Materials Science and Technology 2014 Conference, MS and T 2014 - Pittsburgh, United States
Duration: Oct 12 2014Oct 16 2014

Publication series

NameCeramic Transactions
Volume254
ISSN (Print)1042-1122

Conference

ConferenceNext Generation Biomaterials and Surface Properties of Biomaterials Symposia - Materials Science and Technology 2014 Conference, MS and T 2014
CountryUnited States
CityPittsburgh
Period10/12/1410/16/14

ASJC Scopus subject areas

  • Ceramics and Composites
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Novel PLA- and PCL-HA porous 3D scaffolds prepared by robocasting facilitate MC3T3-E1 subclone 4 cellular attachment and growth'. Together they form a unique fingerprint.

Cite this