Novel embryonic regulation of Ca2+-activated K+ channel expression in Drosophila

Tarita Thomas, B. I.N. Wang, Robert Brenner, Nigel S. Atkinson

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The slowpoke gene of Drosophila melanogaster encodes a Ca2+-activated K+ channel that is expressed in neurons, muscles, tracheal cells and the middle midgut. The entire transcriptional control region of slowpoke is contained in 11 kb of genomic DNA. Previous work has identified four different tissue-specific promoters (Promoters Cl, Clb, Clc and C2) and sequences that regulate their activity. Here we describe and contrast the regulation of neuronal and muscle expression during embryogenesis with its regulation during larval and adult stages. Embryonic regulation is fundamentally different. The embryo uses Promoter Cl and a previously undescribed promoter, called Promoter Ce, to drive neuronal expression. The expression patterns of these promoters are distinct. Muscle expression arises from Promoter C2 as in other developmental stages. A downstream intronic region has been shown to contain control elements that modulate promoter activity differently in embryos, larvae and adults. Embryonic CNS expression is not dependent on the intron, however; its deletion has substantial effects on neuronal expression in larvae and adults. In embryonic muscle, removal of the intron eliminates muscle expression even though this deletion does not reduce larval muscle expression.

Original languageEnglish (US)
Pages (from-to)283-291
Number of pages9
JournalInvertebrate Neuroscience
Volume2
Issue number4
StatePublished - Mar 1997

Keywords

  • Calcium-activated
  • Drosophila
  • Embryo
  • Gene regulation
  • Potassium channel
  • Slowpoke

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

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