Novel cell cycle-related nuclear proteins found in rat and human cells with monoclonal antibodies

A. Black, James W Freeman, G. Zhou, H. Busch

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Monoclonal antibodies which react specifically with the nuclei of interphase cells recognized three nuclear antigens with molecular weights of 110,000 (p110), 85,000 (p85), and 18,000 (p18). p110 and p85 were found in eight tumor cell lines but were not found in resting lymphocytes. p18 was found in resting lymphocytes as well as the tumor cell lines. Protein p85 appeared in phytohemagglutinin-stimulated lymphocytes in the G1 phase and protein p110 appeared in the S phase. p110 and p85 were localized to the extranucleolar chromatin while p18 was distributed throughout the nucleus and was determined by microscopic and DNase digestion studies to be DNA associated. The anti-p110 antibody recognized a component of the DNA polymerase α2 complex. Three novel nuclear proteins were identified using monoclonal antibodies. Two of these proteins (p110 and p85) are proliferating cell nuclear and nucleolar antigen-like while the third (p18) is not cell cycle dependent.

Original languageEnglish (US)
Pages (from-to)3266-3272
Number of pages7
JournalCancer Research
Volume47
Issue number12
StatePublished - 1987
Externally publishedYes

Fingerprint

Nuclear Proteins
Cell Cycle
Monoclonal Antibodies
Lymphocytes
Tumor Cell Line
Nuclear Antigens
Proteins
Deoxyribonucleases
Interphase
Proliferating Cell Nuclear Antigen
G1 Phase
Phytohemagglutinins
DNA-Directed DNA Polymerase
Cell Nucleus
S Phase
Chromatin
Digestion
Anti-Idiotypic Antibodies
Molecular Weight
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Black, A., Freeman, J. W., Zhou, G., & Busch, H. (1987). Novel cell cycle-related nuclear proteins found in rat and human cells with monoclonal antibodies. Cancer Research, 47(12), 3266-3272.

Novel cell cycle-related nuclear proteins found in rat and human cells with monoclonal antibodies. / Black, A.; Freeman, James W; Zhou, G.; Busch, H.

In: Cancer Research, Vol. 47, No. 12, 1987, p. 3266-3272.

Research output: Contribution to journalArticle

Black, A, Freeman, JW, Zhou, G & Busch, H 1987, 'Novel cell cycle-related nuclear proteins found in rat and human cells with monoclonal antibodies', Cancer Research, vol. 47, no. 12, pp. 3266-3272.
Black, A. ; Freeman, James W ; Zhou, G. ; Busch, H. / Novel cell cycle-related nuclear proteins found in rat and human cells with monoclonal antibodies. In: Cancer Research. 1987 ; Vol. 47, No. 12. pp. 3266-3272.
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