Novel asparagine-derived lipid enhances distearoylphosphatidylcholine bilayer resistance to acidic conditions

Adelphe M. Mfuh, Mathew P.D. Mahindaratne, Maritza V. Quintero, Frederick J. Lakner, Ande Bao, Beth A. Goins, William T. Phillips, George R. Negrete

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


A novel asparagine-derived lipid analogue (ALA11,17) bearing a tetrahydropyrimidinone headgroup and two fatty chains (11 and 17 indicate the lengths of linear alkyl groups) was synthesized in high yield and purity. The thin film hydration of formulations containing 5 mol % or greater ALA 11,17 in distearoylphosphatidylcholine (DSPC) generated multilamellar vesicles (MLVs) that remained unaggregated according to optical microscopy, while those formed from DSPC only were highly clustered. The MLVs were processed into unilamellar liposomes via extrusion and were characterized by dynamic light scattering (DLS), zeta potential, turbidity, and scanning electron microscopy (SEM) analysis. Results show that the presence of ALA11,17 in DSPC liposomes significantly alters the morphology, colloidal stability, and retention of encapsulated materials in both acidic and neutral conditions. The ability of ALA11,17-hybrid liposomes to encapsulate and retain inclusions under neutral and acidic conditions (pH < 2) was demonstrated by calcein dequenching experiments. DLS and SEM confirmed that ALA 11,17/DSPC liposomes remained intact under these conditions. The bilayer integrity observed under neutral and acidic conditions and the likely biocompatibility of these fatty amino acid analogues suggest that ALA 11,17 is a promising additive for modulating phosphatidylcholine lipid bilayer properties.

Original languageEnglish (US)
Pages (from-to)4447-4455
Number of pages9
Issue number8
StatePublished - Apr 19 2011

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


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