Normal immune function in young and old DNA polymerase-β deficient mice

Mohammad A. Pahlavani, Daniel M. Vargas, Zhongmao Guo, Arlan Richardson

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The effect of the DNA polymerase-β (β-pol) deficiency on mitogenic response and cytokine production was studied in spleen lymphocytes from 4-5- and 20-22-month-old β-pol(-/+) mice and their age-matched wild-type littermates. The proliferative response of lymphocytes to Concanavalin A (Con A) and lipopolysaccharide (LPS) was measured by [3H]thymidine incorporation, and the induction of cytokine production (interleukin (IL)-2, IL-4, and interferon necrosis factor (IFN)-γ) was assessed by enzyme-linked immunosorbent assay. There was no significant difference in Con A- or LPS-induced proliferation or cytokine production in young β-pol(-/+) mice compared with young wild-type littermates or in old β-pol(-/+) mice compared with old wild-type littermates. However, mitogen-induced proliferation and cytokine production changed significantly with age. The proliferative response to Con A and to LPS, and the IL-2 production was significantly lower, and IL-4 and IFN-γ levels were significantly higher in lymphocytes from old β-pol(-/+) mice and old wild-type mice than in lymphocytes from young β-pol(-/+) mice and young wild-type littermates. In addition, flow cytometric analysis showed no significant differences between young β-pol(-/+) mice and young wild-type littermates or between old β-pol(-/+) mice and old wild-type littermates in the proportion of B- and T-cell populations, and T-cell subsets. However, the number of lymphocytes expressing CD4+ phenotype slightly decreased and the proportion of lymphocytes expressing CD44/Pgp-1 (memory) phenotype increased with age. Thus, we found no evidence for alteration in immune function in DNA polymerase-β deficient mice, although they exhibit a decline in immunologic function with age. Copyright (C) 2000 Elsevier Science S.A.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalImmunology Letters
Volume72
Issue number1
DOIs
StatePublished - Apr 3 2000

Keywords

  • Aging
  • DNA polymerase-β
  • DNA repair
  • Immune function
  • Knockout mice

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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