Noncoordinate control of RNA synthesis in eucaryotic cells

Mary V. Willis, Joel B. Baseman, Harold Amos

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Inhibition of protein synthesis in confluent monolayers of chick fibroblasts stimulates selectively the synthesis of 4S RNA, resulting in a net accumulation of 4S RNA in the inhibited cells. Under these conditions, inhibition of ribosomal RNA synthesis and processing occurs, as does a decrease in soluble uridine phosphate concentrations; increased pools of certain amino acids are also apparent. Recovery of cells from inhibition is accompanied by a rapidly increasing rate of protein synthesis that lasts for several hours. The small molecular weight RNA synthesized during inhibition of protein synthesis appears properly methylated, and in the presence of cycloheximide and actinomycin D shows a precursor-product conversion. Radiolabeled RNA synthesized during inhibition of protein synthesis is stable following the recovery of cells from inhibition. Stimulation of uridine incorporation into 4S RNA during arrest of protein synthesis is also demonstrated in high-density cultures of L- and Hep-2 cells, suggesting that this non-coordinate stimulation of 4S RNA may be a general property of eucaryotic cells.

Original languageEnglish (US)
Pages (from-to)179-184
Number of pages6
JournalCell
Volume3
Issue number2
DOIs
StatePublished - 1974
Externally publishedYes

Fingerprint

RNA
Proteins
Uracil Nucleotides
Small Nuclear RNA
Ribosomal RNA
Uridine
Dactinomycin
Cycloheximide
Recovery
Fibroblasts
Amino Acids
Monolayers
Processing

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Noncoordinate control of RNA synthesis in eucaryotic cells. / Willis, Mary V.; Baseman, Joel B.; Amos, Harold.

In: Cell, Vol. 3, No. 2, 1974, p. 179-184.

Research output: Contribution to journalArticle

Willis, MV, Baseman, JB & Amos, H 1974, 'Noncoordinate control of RNA synthesis in eucaryotic cells', Cell, vol. 3, no. 2, pp. 179-184. https://doi.org/10.1016/0092-8674(74)90123-8
Willis, Mary V. ; Baseman, Joel B. ; Amos, Harold. / Noncoordinate control of RNA synthesis in eucaryotic cells. In: Cell. 1974 ; Vol. 3, No. 2. pp. 179-184.
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