TY - JOUR
T1 - Non-denaturing gel electrophoresis of biological nanoparticles
T2 - viruses
AU - Serwer, Philip
AU - Khan, Saeed A.
AU - Griess, Gary A.
N1 - Funding Information:
The authors thank Linda C. Winchester for typing the manuscript. Work in the authors' laboratory was supported by the US National Science Foundation (BIR 9003695 and MCB 9316660), the National Instituteso f Health (GM
PY - 1995/4/28
Y1 - 1995/4/28
N2 - Although gel electrophoresis is usually used for the fractionation of monomolecular particles, it is also applicable to the fractionation of the multimolecular complexes produced during both cellular metabolism and assembly of viruses in virus-infected cells. Gel electrophoretic procedures have been developed for determining both the size of a spherical particle and some aspects of the shape of a non-spherical particle. Capsids bound to DNA outside of the capsid can also be both fractionated and characterized. The procedures developed will be used for screening currl mutants; they also can pe used for diagnostic virology. Sensitivity of detection, the major current limitation, is being improved by use of both improved stains and scanning fluorimetry. The gels used for fractionation sometimes approximate random straight fiber gels, but become increasingly biphasic as the gel concentration is decreased.
AB - Although gel electrophoresis is usually used for the fractionation of monomolecular particles, it is also applicable to the fractionation of the multimolecular complexes produced during both cellular metabolism and assembly of viruses in virus-infected cells. Gel electrophoretic procedures have been developed for determining both the size of a spherical particle and some aspects of the shape of a non-spherical particle. Capsids bound to DNA outside of the capsid can also be both fractionated and characterized. The procedures developed will be used for screening currl mutants; they also can pe used for diagnostic virology. Sensitivity of detection, the major current limitation, is being improved by use of both improved stains and scanning fluorimetry. The gels used for fractionation sometimes approximate random straight fiber gels, but become increasingly biphasic as the gel concentration is decreased.
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U2 - 10.1016/0021-9673(94)01259-H
DO - 10.1016/0021-9673(94)01259-H
M3 - Review article
C2 - 7773365
AN - SCOPUS:0028947389
VL - 698
SP - 251
EP - 261
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -