No evidence of infection with porcine endogenous retrovirus in recipients of porcine islet-cell xenografts

Walid Heneine, Annika Tibell, William M. Switzer, Paul Sandstrom, Guillermo Vazquez Resales, Aprille Mathews, Olle Korsgren, Louisa E. Chapman, Thomas M. Folks, Carl G. Groth

    Research output: Contribution to journalArticlepeer-review

    306 Scopus citations

    Abstract

    Background. The study of whether porcine xenografts can lead to porcine endogenous retrovirus (PERV) infection of recipients is critical for evaluating the safety of pig-to-man xenotransplantation. PERV is carried in the pig germline, and all recipients of porcine tissues or organs will be exposed to the virus. Methods. We studied 10 diabetic patients who had received porcine fetal islets between 1990 and 1993, looking for evidence of PERV infection by using PCR serology, PCR, and reverse transcriptase assays. Prolonged xenograft survival (up to a year) was confirmed in five patients by porcine C-peptide excretion and detection of pig mitochondrial DNA (mtDNA) in serum. Findings. Despite the evidence for extended exposure to pig cells and despite concomitant immunosuppressive therapy, we were unable to detect markers of PERV infection in any patient. Screening for two PERV sequences in peripheral blood lymphocytes collected 4-7 years after the xenotransplantation was negative. Markers of PERV expression, including viral RNA and reverse transcriptase, were undetectable in sera from both early (day 3 to day 180) and late (4-7 years) time points. Western blot analysis for antibodies was consistently negative. Interpretation. These results suggested the absence of PERV infection in these patients. Also this study establishes a minimum standard for post-transplant surveillance of patients given porcine xenografts.

    Original languageEnglish (US)
    Pages (from-to)695-699
    Number of pages5
    JournalLancet
    Volume352
    Issue number9129
    DOIs
    StatePublished - Aug 29 1998

    ASJC Scopus subject areas

    • Medicine(all)

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