N,N′-o-Phenylenedi[1,4-14C]maleimide. its synthesis and application in the cross-linking of Escherichia coli succinic thiokinase

Jonathan S. Nishimura, James A. Petrich, Albert F. Milne, Theresa Mitchell

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

1. 1. The syntheses of N,N′-o-phenylenedi[1,4-14C]maleimide (60-70% yield) and the unlabeled ortho and para isomers (40-50% yield) have been carried out on a milligram scale. 2. 2. Use of the 14C-labeled compound with Escherichia coli succinic thiokinase showed that the crosslinking agent combined with the β subunit first in the formation of αβ dimer. Incorporation of about 2.5 moles of the reagent (40% of this bound bifunctionally) was sufficient to inhibit enzyme activity completely.

Original languageEnglish (US)
Pages (from-to)93-96
Number of pages4
JournalInternational Journal of Biochemistry
Volume9
Issue number2
DOIs
StatePublished - 1978
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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