N,N-Bis(α-iodoacetyl)-2,2′-dithiobis(ethylamine), a reversible crosslinking reagent for protein sulfhydryl groups

Richard F. Ludueña, Mary Carmen Roach, Phyllis P. Trcka, Susan Weintraub

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

This paper describes the synthesis and analysis of N,N′-bis(α-iodoacetyl)-2-2′-dithiobis(ethylamine) (BIDBE), a bifunctional reagent with the structure ICH2CONH-(CH2)2SS(CH2)2NHCOCH2I. BIDBE is shown to react with the sulfhydryl groups of tubulin and to generate a cleavable crosslink between aldolase subunits. BIDBE may be the only reagent hitherto described which can generate a cleavable crosslink between nonadjacent sulfhydryl groups in proteins.

Original languageEnglish (US)
Pages (from-to)76-80
Number of pages5
JournalAnalytical Biochemistry
Volume117
Issue number1
DOIs
StatePublished - Oct 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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