N,N-Bis(α-iodoacetyl)-2,2′-dithiobis(ethylamine), a reversible crosslinking reagent for protein sulfhydryl groups

Richard F. Ludueña; Mary Carmen Roach; Phyllis P. Trcka; Susan Weintraub

doi:10.1016/0003-2697(81)90694-1

N,N-Bis(α-iodoacetyl)-2,2′-dithiobis(ethylamine), a reversible crosslinking reagent for protein sulfhydryl groups

Richard F. Ludueña, Mary Carmen Roach, Phyllis P. Trcka, Susan Weintraub

Department of Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

This paper describes the synthesis and analysis of N,N′-bis(α-iodoacetyl)-2-2′-dithiobis(ethylamine) (BIDBE), a bifunctional reagent with the structure ICH₂CONH-(CH₂)₂SS(CH₂)₂NHCOCH₂I. BIDBE is shown to react with the sulfhydryl groups of tubulin and to generate a cleavable crosslink between aldolase subunits. BIDBE may be the only reagent hitherto described which can generate a cleavable crosslink between nonadjacent sulfhydryl groups in proteins.

Original language	English (US)
Pages (from-to)	76-80
Number of pages	5
Journal	Analytical Biochemistry
Volume	117
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(81)90694-1
State	Published - Oct 1981

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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title = "N,N-Bis(α-iodoacetyl)-2,2′-dithiobis(ethylamine), a reversible crosslinking reagent for protein sulfhydryl groups",

abstract = "This paper describes the synthesis and analysis of N,N′-bis(α-iodoacetyl)-2-2′-dithiobis(ethylamine) (BIDBE), a bifunctional reagent with the structure ICH2CONH-(CH2)2SS(CH2)2NHCOCH2I. BIDBE is shown to react with the sulfhydryl groups of tubulin and to generate a cleavable crosslink between aldolase subunits. BIDBE may be the only reagent hitherto described which can generate a cleavable crosslink between nonadjacent sulfhydryl groups in proteins.",

author = "Ludue{\~n}a, {Richard F.} and Roach, {Mary Carmen} and Trcka, {Phyllis P.} and Susan Weintraub",

note = "Funding Information: We are grateful to R. W. Keenan for his advice and to D. J. Hanahan for his gift of rabbit muscle aldolase. This work was supported by Grant GM 23476 to R. F. Luduefia from the National Institutes of Health.",

year = "1981",

month = oct,

doi = "10.1016/0003-2697(81)90694-1",

language = "English (US)",

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pages = "76--80",

journal = "Analytical Biochemistry",

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T1 - N,N-Bis(α-iodoacetyl)-2,2′-dithiobis(ethylamine), a reversible crosslinking reagent for protein sulfhydryl groups

AU - Ludueña, Richard F.

AU - Roach, Mary Carmen

AU - Trcka, Phyllis P.

AU - Weintraub, Susan

N1 - Funding Information: We are grateful to R. W. Keenan for his advice and to D. J. Hanahan for his gift of rabbit muscle aldolase. This work was supported by Grant GM 23476 to R. F. Luduefia from the National Institutes of Health.

PY - 1981/10

Y1 - 1981/10

N2 - This paper describes the synthesis and analysis of N,N′-bis(α-iodoacetyl)-2-2′-dithiobis(ethylamine) (BIDBE), a bifunctional reagent with the structure ICH2CONH-(CH2)2SS(CH2)2NHCOCH2I. BIDBE is shown to react with the sulfhydryl groups of tubulin and to generate a cleavable crosslink between aldolase subunits. BIDBE may be the only reagent hitherto described which can generate a cleavable crosslink between nonadjacent sulfhydryl groups in proteins.

AB - This paper describes the synthesis and analysis of N,N′-bis(α-iodoacetyl)-2-2′-dithiobis(ethylamine) (BIDBE), a bifunctional reagent with the structure ICH2CONH-(CH2)2SS(CH2)2NHCOCH2I. BIDBE is shown to react with the sulfhydryl groups of tubulin and to generate a cleavable crosslink between aldolase subunits. BIDBE may be the only reagent hitherto described which can generate a cleavable crosslink between nonadjacent sulfhydryl groups in proteins.

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N,N-Bis(α-iodoacetyl)-2,2′-dithiobis(ethylamine), a reversible crosslinking reagent for protein sulfhydryl groups

Abstract

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