NLRP3 is a critical regulator of inflammation and innate immune cell response during Mycoplasma pneumoniae infection

Jesus A. Segovia, Te Hung Chang, Vicki T. Winter, Jacqueline J. Coalson, Marianna P. Cagle, Lavanya Pandranki, Santanu Bose, Joel B. Baseman, Thirumalai R. Kannan

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Mycoplasma pneumoniae is an atypical bacterial respiratory pathogen known to cause a range of airway inflammation and lung and extrapulmonary pathologies. We recently reported that an M. pneumoniae-derived ADP-ribosylating and vacuolating toxin called community-acquired respiratory distress syndrome (CARDS) toxin is capable of triggering NLRP3 (NLR-family, leucine-rich repeat protein 3) inflammasome activation and interleukin-1β (IL-1β) secretion in macrophages. However, it is unclear whether the NLRP3 inflammasome is important for the immune response during M. pneumoniae acute infection. In the current study, we utilized in vitro and in vivo models of M. pneumoniae infection to characterize the role of the NLRP3 inflammasome during acute infection. M. pneumoniae-infected macrophages deficient for inflammasome components NLRP3, ASC (apoptosis speck-like protein containing a caspase activation and recruitment domain), or caspase-1 failed to process and secrete IL-1β. The MyD88/NF-κB signaling pathway was found to be critical for proinflammatory gene expression in macrophages infected with M. pneumoniae. C57BL/6 mice deficient for NLRP3 expression were unable to produce IL-1β in the airways during acute infection, and lack of this inflammatory response led to deficient immune cell activation and delayed bacterial clearance. These findings are the first to report the importance of the NLRP3 inflammasome in regulating the inflammatory response and influencing the progression of M. pneumoniae during acute infection.

Original languageEnglish (US)
Article numbere00548-17
JournalInfection and immunity
Volume86
Issue number1
DOIs
StatePublished - Jan 1 2018

Keywords

  • ADP-ribosylation
  • CARDS toxin
  • Inflammasome
  • Interleukin-1β
  • Mycoplasma pneumoniae
  • NLRP3

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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