Nitric oxide synthase activity in umbilical and placental vascular tissue of gestational diabetic pregnancies

Shaul Dollberg, Diane E. Brockman, Leslie Myatt

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: Nitric oxide (NO) synthase activity in the fetal-placental vasculature of gestational diabetic pregnancies was compared to non-diabetic controls. Methods: Placentae were collected from non-diabetic deliveries (n = 5) and from patients with gestational diabetes (n = 8). Umbilical cord and chorionic plate arteries and veins and stem villous vessels were quickly dissected out, cleaned of contaminating tissue, snap frozen in liquid nitrogen and stored at -80°C. NO synthase activity was measured in the homogenate of these vessels by the arginine to citrulline conversion assay using 5 μM 3H-L-arginine with 1 mM NADPH, 100 μM free calcium, 50 units/ml calmodulin, 10 μM tetrahydrobiopterin and 2 μM flavin adenine dinucleotide with 45-min incubation at 27°C. Enzyme activity was expressed as picomoles of 3H-L-citrulline formed per milligram of protein per minute. Results: No significant differences in NO synthase activity were found between non-diabetic and gestational diabetics for umbilical cord artery (0.58 ± 0.22 vs. 0.19 ± 0.06), cord vein (0.39 ± 0.21 vs. 0.07 ± 0.03), chorionic plate artery (0.32 ± 0.14 vs. 0.26 ± 0.19) or vein (0.41 ± 0.15 vs. 0.22 ± 0.06), respectively (mean ± SEM). Significantly greater activity was found in stem villous vessels of non-diabetic placentae (5.64 ± 2.0) compared to those of gestational diabetic placentae (0.48 ± 0.19; p < 0.01). Conclusion: The reduced blood flow and increased vascular resistance observed in diabetic pregnancies may be due to decreased NO synthesis and activity in the stem villous vessels which are the major determinants of resistance in the fetal-placental vasculature.

Original languageEnglish (US)
Pages (from-to)177-181
Number of pages5
JournalGynecologic and Obstetric Investigation
Volume44
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

Umbilicus
Nitric Oxide Synthase
Placenta
Blood Vessels
Veins
Citrulline
Umbilical Cord
Pregnancy
Arginine
Arteries
Pregnancy in Diabetics
Fetal Movement
Umbilical Arteries
Flavin-Adenine Dinucleotide
Gestational Diabetes
Calmodulin
NADP
Vascular Resistance
Nitric Oxide
Nitrogen

Keywords

  • Gestational diabetes
  • Nitric oxide synthase
  • Villous vascular tree

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Nitric oxide synthase activity in umbilical and placental vascular tissue of gestational diabetic pregnancies. / Dollberg, Shaul; Brockman, Diane E.; Myatt, Leslie.

In: Gynecologic and Obstetric Investigation, Vol. 44, No. 3, 1997, p. 177-181.

Research output: Contribution to journalArticle

Dollberg, Shaul ; Brockman, Diane E. ; Myatt, Leslie. / Nitric oxide synthase activity in umbilical and placental vascular tissue of gestational diabetic pregnancies. In: Gynecologic and Obstetric Investigation. 1997 ; Vol. 44, No. 3. pp. 177-181.
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AB - Objective: Nitric oxide (NO) synthase activity in the fetal-placental vasculature of gestational diabetic pregnancies was compared to non-diabetic controls. Methods: Placentae were collected from non-diabetic deliveries (n = 5) and from patients with gestational diabetes (n = 8). Umbilical cord and chorionic plate arteries and veins and stem villous vessels were quickly dissected out, cleaned of contaminating tissue, snap frozen in liquid nitrogen and stored at -80°C. NO synthase activity was measured in the homogenate of these vessels by the arginine to citrulline conversion assay using 5 μM 3H-L-arginine with 1 mM NADPH, 100 μM free calcium, 50 units/ml calmodulin, 10 μM tetrahydrobiopterin and 2 μM flavin adenine dinucleotide with 45-min incubation at 27°C. Enzyme activity was expressed as picomoles of 3H-L-citrulline formed per milligram of protein per minute. Results: No significant differences in NO synthase activity were found between non-diabetic and gestational diabetics for umbilical cord artery (0.58 ± 0.22 vs. 0.19 ± 0.06), cord vein (0.39 ± 0.21 vs. 0.07 ± 0.03), chorionic plate artery (0.32 ± 0.14 vs. 0.26 ± 0.19) or vein (0.41 ± 0.15 vs. 0.22 ± 0.06), respectively (mean ± SEM). Significantly greater activity was found in stem villous vessels of non-diabetic placentae (5.64 ± 2.0) compared to those of gestational diabetic placentae (0.48 ± 0.19; p < 0.01). Conclusion: The reduced blood flow and increased vascular resistance observed in diabetic pregnancies may be due to decreased NO synthesis and activity in the stem villous vessels which are the major determinants of resistance in the fetal-placental vasculature.

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