Nitric oxide prevents anoxia-induced apoptosis in colonic HT29 cells

Muniswamy Madesh, Anup Ramachandran, K. A. Balasubramanian

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Apoptosis is a critical determinant of tissue mass homeostasis and may play a role in carcinogenesis. The aim of the present study was to investigate anoxia-induced cell death in colon-derived HT29 cells and the effect of nitric oxide on this phenomenon. It was found that HT29 cells subjected to anoxia undergo apoptosis in a time dependent manner, as determined by DNA fragmentation and Hoechst-33258 dye staining. Cytochrome c release from mitochondria to cytosol is a key step in this process and this release precedes DNA fragmentation. NO inhibits anoxia induced apoptosis in these cells by inhibiting the release of cytochrome c and thus may play a role in modulating the apoptotic cell death of colon-derived epithelial cells.

Original languageEnglish (US)
Pages (from-to)240-248
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume366
Issue number2
DOIs
StatePublished - Jun 15 1999
Externally publishedYes

Keywords

  • Apoptosis
  • Cytochrome c
  • HT29 cells
  • Nitric oxide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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