Nitric oxide prevents anoxia-induced apoptosis in colonic HT29 cells

Muniswamy Madesh; Anup Ramachandran; K. A. Balasubramanian

doi:10.1006/abbi.1999.1185

Nitric oxide prevents anoxia-induced apoptosis in colonic HT29 cells

Muniswamy Madesh, Anup Ramachandran, K. A. Balasubramanian

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Apoptosis is a critical determinant of tissue mass homeostasis and may play a role in carcinogenesis. The aim of the present study was to investigate anoxia-induced cell death in colon-derived HT29 cells and the effect of nitric oxide on this phenomenon. It was found that HT29 cells subjected to anoxia undergo apoptosis in a time dependent manner, as determined by DNA fragmentation and Hoechst-33258 dye staining. Cytochrome c release from mitochondria to cytosol is a key step in this process and this release precedes DNA fragmentation. NO inhibits anoxia induced apoptosis in these cells by inhibiting the release of cytochrome c and thus may play a role in modulating the apoptotic cell death of colon-derived epithelial cells.

Original language	English (US)
Pages (from-to)	240-248
Number of pages	9
Journal	Archives of Biochemistry and Biophysics
Volume	366
Issue number	2
DOIs	https://doi.org/10.1006/abbi.1999.1185
State	Published - Jun 15 1999
Externally published	Yes

Keywords

Apoptosis
Cytochrome c
HT29 cells
Nitric oxide

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1006/abbi.1999.1185

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title = "Nitric oxide prevents anoxia-induced apoptosis in colonic HT29 cells",

abstract = "Apoptosis is a critical determinant of tissue mass homeostasis and may play a role in carcinogenesis. The aim of the present study was to investigate anoxia-induced cell death in colon-derived HT29 cells and the effect of nitric oxide on this phenomenon. It was found that HT29 cells subjected to anoxia undergo apoptosis in a time dependent manner, as determined by DNA fragmentation and Hoechst-33258 dye staining. Cytochrome c release from mitochondria to cytosol is a key step in this process and this release precedes DNA fragmentation. NO inhibits anoxia induced apoptosis in these cells by inhibiting the release of cytochrome c and thus may play a role in modulating the apoptotic cell death of colon-derived epithelial cells.",

keywords = "Apoptosis, Cytochrome c, HT29 cells, Nitric oxide",

author = "Muniswamy Madesh and Anup Ramachandran and Balasubramanian, {K. A.}",

note = "Funding Information: The Wellcome Trust Research Laboratory is supported by The Wellcome Trust, London. Financial assistance from the Department of Science and Technology, Government of India, is gratefully acknowledged. M. Madesh (SRF) and Anup R. (SRF) are recipients of a fellowship of the Council of Scientific and Industrial Research, India. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

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AU - Madesh, Muniswamy

AU - Ramachandran, Anup

AU - Balasubramanian, K. A.

N1 - Funding Information: The Wellcome Trust Research Laboratory is supported by The Wellcome Trust, London. Financial assistance from the Department of Science and Technology, Government of India, is gratefully acknowledged. M. Madesh (SRF) and Anup R. (SRF) are recipients of a fellowship of the Council of Scientific and Industrial Research, India. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 1999/6/15

Y1 - 1999/6/15

N2 - Apoptosis is a critical determinant of tissue mass homeostasis and may play a role in carcinogenesis. The aim of the present study was to investigate anoxia-induced cell death in colon-derived HT29 cells and the effect of nitric oxide on this phenomenon. It was found that HT29 cells subjected to anoxia undergo apoptosis in a time dependent manner, as determined by DNA fragmentation and Hoechst-33258 dye staining. Cytochrome c release from mitochondria to cytosol is a key step in this process and this release precedes DNA fragmentation. NO inhibits anoxia induced apoptosis in these cells by inhibiting the release of cytochrome c and thus may play a role in modulating the apoptotic cell death of colon-derived epithelial cells.

AB - Apoptosis is a critical determinant of tissue mass homeostasis and may play a role in carcinogenesis. The aim of the present study was to investigate anoxia-induced cell death in colon-derived HT29 cells and the effect of nitric oxide on this phenomenon. It was found that HT29 cells subjected to anoxia undergo apoptosis in a time dependent manner, as determined by DNA fragmentation and Hoechst-33258 dye staining. Cytochrome c release from mitochondria to cytosol is a key step in this process and this release precedes DNA fragmentation. NO inhibits anoxia induced apoptosis in these cells by inhibiting the release of cytochrome c and thus may play a role in modulating the apoptotic cell death of colon-derived epithelial cells.

KW - Apoptosis

KW - Cytochrome c

KW - HT29 cells

KW - Nitric oxide

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Nitric oxide prevents anoxia-induced apoptosis in colonic HT29 cells

Abstract

Keywords

ASJC Scopus subject areas

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