Nitric oxide modulates Ca2+ channel currents in rat sympathetic neurons

Chu Chen; Geoffrey G. Schofield

doi:10.1016/0014-2999(93)90171-D

Nitric oxide modulates Ca²⁺ channel currents in rat sympathetic neurons

Chu Chen, Geoffrey G. Schofield

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

The effects of nitric oxide on the Ca²⁺ channel currents of adult rat superior cervical ganglion neurons were investigated using the whole-cell patch-clamp technique. Nitric oxide-containing compounds sodium nitroprusside and S-nitroso-N-acetyl-penicillamine increased the amplitude of Ca²⁺ currents by intracellular application. In addition, both sodium nitroprusside and S-nitroso-N-acetylpenicillamine reduced norepinephrine-induced inhibition of Ca²⁺ currents. The results suggest that nitric oxide might be involved in the modulation of Ca²⁺-dependent neurotransmission in sympathetic neurons.

Original language	English (US)
Pages (from-to)	83-86
Number of pages	4
Journal	European Journal of Pharmacology
Volume	243
Issue number	1
DOIs	https://doi.org/10.1016/0014-2999(93)90171-D
State	Published - Oct 12 1993
Externally published	Yes

Keywords

Ca channels
Nitric oxide (NO)
Norepinephrine
Patch-clamp
Sympathetic ganglion
Voltage-clamp

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/0014-2999(93)90171-D

Cite this

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title = "Nitric oxide modulates Ca2+ channel currents in rat sympathetic neurons",

abstract = "The effects of nitric oxide on the Ca2+ channel currents of adult rat superior cervical ganglion neurons were investigated using the whole-cell patch-clamp technique. Nitric oxide-containing compounds sodium nitroprusside and S-nitroso-N-acetyl-penicillamine increased the amplitude of Ca2+ currents by intracellular application. In addition, both sodium nitroprusside and S-nitroso-N-acetylpenicillamine reduced norepinephrine-induced inhibition of Ca2+ currents. The results suggest that nitric oxide might be involved in the modulation of Ca2+-dependent neurotransmission in sympathetic neurons.",

keywords = "Ca channels, Nitric oxide (NO), Norepinephrine, Patch-clamp, Sympathetic ganglion, Voltage-clamp",

author = "Chu Chen and Schofield, {Geoffrey G.}",

note = "Funding Information: This work was supported by U S. P H S. grant HL-43656 We are grateful to Dr. P J. Kadowltz and L J Ignarro for the kind gift of S-nltroso-N-acetylpeniclllamme and Dr S R Ikeda for the data acquisition and analysis programs",

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doi = "10.1016/0014-2999(93)90171-D",

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T1 - Nitric oxide modulates Ca2+ channel currents in rat sympathetic neurons

AU - Chen, Chu

AU - Schofield, Geoffrey G.

N1 - Funding Information: This work was supported by U S. P H S. grant HL-43656 We are grateful to Dr. P J. Kadowltz and L J Ignarro for the kind gift of S-nltroso-N-acetylpeniclllamme and Dr S R Ikeda for the data acquisition and analysis programs

PY - 1993/10/12

Y1 - 1993/10/12

N2 - The effects of nitric oxide on the Ca2+ channel currents of adult rat superior cervical ganglion neurons were investigated using the whole-cell patch-clamp technique. Nitric oxide-containing compounds sodium nitroprusside and S-nitroso-N-acetyl-penicillamine increased the amplitude of Ca2+ currents by intracellular application. In addition, both sodium nitroprusside and S-nitroso-N-acetylpenicillamine reduced norepinephrine-induced inhibition of Ca2+ currents. The results suggest that nitric oxide might be involved in the modulation of Ca2+-dependent neurotransmission in sympathetic neurons.

AB - The effects of nitric oxide on the Ca2+ channel currents of adult rat superior cervical ganglion neurons were investigated using the whole-cell patch-clamp technique. Nitric oxide-containing compounds sodium nitroprusside and S-nitroso-N-acetyl-penicillamine increased the amplitude of Ca2+ currents by intracellular application. In addition, both sodium nitroprusside and S-nitroso-N-acetylpenicillamine reduced norepinephrine-induced inhibition of Ca2+ currents. The results suggest that nitric oxide might be involved in the modulation of Ca2+-dependent neurotransmission in sympathetic neurons.

KW - Ca channels

KW - Nitric oxide (NO)

KW - Norepinephrine

KW - Patch-clamp

KW - Sympathetic ganglion

KW - Voltage-clamp

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