Abstract
Previous experiments from our laboratory have indicated that LHRH release is controlled in vivo and in vitro by NO. Since glutamic acid, the major excitatory transmitter in the brain, has been shown to release LHRH, we wished to determine whether or not this LHRH release was mediated by NO. Consequently, arcuate-median eminence explants from normal male rats were incubated in vitro in Krebs-Ringer bicarbonate glucose (KRBG) media in a Dubnoff metabolic shaker for a preincubation period of 30 min. Fresh media were added containing the substances to be tested and incubation was continued for 30 min. Sodium nitroprusside (NP, 500 μM), which releases NO spontaneously, stimulated the release of LHRH, which indicates that NO can release LHRH. Glutamic acid (10 mM) also produced a robust release of LHRH, and this release was blocked by the inhibitor of NO synthase, NG-monomethyl-L-arginine (NMMA). Furthermore, the release of LHRH induced by glutamic acid was prevented by the addition of hemoglobin (20 βg/ml), a scavenger of NO, which would remove the NO released by the action of glutamic acid. The results indicate that glutamic acid stimulated LHRH release is induced by NO.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 501-503 |
| Number of pages | 3 |
| Journal | Brain Research Bulletin |
| Volume | 33 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1994 |
| Externally published | Yes |
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Keywords
- Arcuate-median expiants
- Hemoglobin
- N-monomethyl-L-arginine
- Nitric oxide synthase
- Nitroprusside
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Nitric oxide mediates the stimulation of luteinizing-hormone releasing hormone release induced by glutamic acid in vitro. / Rettori, Valeria; Kamat, Amrita; McCann, Samuel M.
In: Brain Research Bulletin, Vol. 33, No. 5, 1994, p. 501-503.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nitric oxide mediates the stimulation of luteinizing-hormone releasing hormone release induced by glutamic acid in vitro
AU - Rettori, Valeria
AU - Kamat, Amrita
AU - McCann, Samuel M.
PY - 1994
Y1 - 1994
N2 - Previous experiments from our laboratory have indicated that LHRH release is controlled in vivo and in vitro by NO. Since glutamic acid, the major excitatory transmitter in the brain, has been shown to release LHRH, we wished to determine whether or not this LHRH release was mediated by NO. Consequently, arcuate-median eminence explants from normal male rats were incubated in vitro in Krebs-Ringer bicarbonate glucose (KRBG) media in a Dubnoff metabolic shaker for a preincubation period of 30 min. Fresh media were added containing the substances to be tested and incubation was continued for 30 min. Sodium nitroprusside (NP, 500 μM), which releases NO spontaneously, stimulated the release of LHRH, which indicates that NO can release LHRH. Glutamic acid (10 mM) also produced a robust release of LHRH, and this release was blocked by the inhibitor of NO synthase, NG-monomethyl-L-arginine (NMMA). Furthermore, the release of LHRH induced by glutamic acid was prevented by the addition of hemoglobin (20 βg/ml), a scavenger of NO, which would remove the NO released by the action of glutamic acid. The results indicate that glutamic acid stimulated LHRH release is induced by NO.
AB - Previous experiments from our laboratory have indicated that LHRH release is controlled in vivo and in vitro by NO. Since glutamic acid, the major excitatory transmitter in the brain, has been shown to release LHRH, we wished to determine whether or not this LHRH release was mediated by NO. Consequently, arcuate-median eminence explants from normal male rats were incubated in vitro in Krebs-Ringer bicarbonate glucose (KRBG) media in a Dubnoff metabolic shaker for a preincubation period of 30 min. Fresh media were added containing the substances to be tested and incubation was continued for 30 min. Sodium nitroprusside (NP, 500 μM), which releases NO spontaneously, stimulated the release of LHRH, which indicates that NO can release LHRH. Glutamic acid (10 mM) also produced a robust release of LHRH, and this release was blocked by the inhibitor of NO synthase, NG-monomethyl-L-arginine (NMMA). Furthermore, the release of LHRH induced by glutamic acid was prevented by the addition of hemoglobin (20 βg/ml), a scavenger of NO, which would remove the NO released by the action of glutamic acid. The results indicate that glutamic acid stimulated LHRH release is induced by NO.
KW - Arcuate-median expiants
KW - Hemoglobin
KW - N-monomethyl-L-arginine
KW - Nitric oxide synthase
KW - Nitroprusside
UR - http://www.scopus.com/inward/record.url?scp=0028355420&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028355420&partnerID=8YFLogxK
U2 - 10.1016/0361-9230(94)90074-4
DO - 10.1016/0361-9230(94)90074-4
M3 - Article
C2 - 7514483
AN - SCOPUS:0028355420
VL - 33
SP - 501
EP - 503
JO - Brain Research Bulletin
JF - Brain Research Bulletin
SN - 0361-9230
IS - 5
ER -