NGF Up-regulates TRPA1: Implications for orofacial pain

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The transient receptor potential ankyrin repeat 1 (TRPA1) channel is believed to be involved in many forms of acute and chronic hyperalgesia. Nerve Growth Factor (NGF) regulates chronic inflammatory hyperalgesia by controlling gene expression in sensory neurons, including genes involved in inflammatory hyperalgesia in the dental pulp. We hypothesized that NGF increases functional activities of the TRPA1 channel in trigeminal ganglion neurons. Here, we show that NGF induced a concentration- and time-dependent up-regulation of TRPA1 mRNA in trigeminal ganglia neurons, as detected by real-time RT-PCR and in situ hybridization. In addition, NGF evoked a time-dependent increase of mustard oil (MO)evoked TRPA1 activation in trigeminal ganglia neurons. Collectively, these findings demonstrate that NGF participates in the functional upregulation of TRPA1 in trigeminal ganglia neurons. These enhanced activities of TRPA1 could play an important role in the development of hyperalgesia following nerve injury and inflammation in the orofacial region.

Original languageEnglish (US)
Pages (from-to)550-555
Number of pages6
JournalJournal of Dental Research
Volume86
Issue number6
DOIs
StatePublished - Jun 2007

Fingerprint

Ankyrin Repeat
Facial Pain
Nerve Growth Factor
Trigeminal Ganglion
Up-Regulation
Hyperalgesia
Neurons
Dental Pulp
Sensory Receptor Cells
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Inflammation
Gene Expression
Messenger RNA
Wounds and Injuries
Genes

Keywords

  • NGF
  • Pain
  • RT-PCR
  • Trigeminal
  • TRPA1

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

NGF Up-regulates TRPA1 : Implications for orofacial pain. / Diogenes, Anibal R; Akopian, Armen N; Hargreaves, Kenneth M.

In: Journal of Dental Research, Vol. 86, No. 6, 06.2007, p. 550-555.

Research output: Contribution to journalArticle

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