NF-κB DNA-Binding Activity after High Peak Power Pulsed Microwave (8.2 GHz) Exposure of Normal Human Monocytes

Mohan Natarajan, Vilayalaxmi, Maria Szilagyl, Francis N. Roldan, Martin L. Meltz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The hypothesis investigated is that exposure of a mammalian cell to high peak power pulsed RF, at the frequency of 8.2 GHz, can result in the activation of an important eukaryotic transcriptional regulator, nuclear factor kappa B (NF-κB). This DNA-binding protein controls genes involved in long term cellular regulation. The selection of 8.2 GHz was based on the availability of a high peak power pulsed RF transmitter. In these studies, triplicate cultures of human monocytes (Mono Mac-6) were exposed to the pulsed wave radiation. The peak to average power ratio was 455:1 (2.2 μs pulse width and pulse repetition rate of 1000 pulses/s). The average power density at the position of exposure was 50 W/m2, and the mean SAR at the bottom of the culture flask was 10.8 ± 7.1 W/kg. The FDTD analysis indicated that 10% of the cells had an SAR of 22-29 W/kg. The cells were exposed continuously for 90 min at 37 °C, reincubated at this temperature, and harvested 4 h postexposure. The nuclear extracts were analyzed by electrophoretic mobility shift assay. The results showed a profound increase (3.6-fold) in the DNA binding activity of NF-κB in monocytes at 4 h after the pulsed RF exposure compared to sham irradiated controls. Competition experiments with cold NF-κB- specific oligonucleotides confirmed the specificity of the DNA binding activity. These results provide evidence that high peak power pulsed radiofrequency radiation can perturb the cell and initiate cell signaling pathways. However, at this point, we are not prepared to advocate that the cause is a nonthermal mechanism. Because of the broad distribution of SAR's in the flask, experiments need to be performed to determine if the changes observed are associated with cells exposed to high or low SARs.

Original languageEnglish (US)
Pages (from-to)271-277
Number of pages7
JournalBioelectromagnetics
Volume23
Issue number4
DOIs
StatePublished - May 2002

Fingerprint

B-DNA
NF-kappa B
Microwaves
monocytes
Monocytes
DNA
cells
Radiation
DNA-binding proteins
DNA-Binding Proteins
Electrophoretic Mobility Shift Assay
oligonucleotides
Oligonucleotides
heart rate
transcription factors
Heart Rate
Temperature
extracts
assays
Genes

Keywords

  • Cellular responses
  • Mono mac-6 cells
  • Radiofrequency radiation
  • Transcription factor

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics

Cite this

NF-κB DNA-Binding Activity after High Peak Power Pulsed Microwave (8.2 GHz) Exposure of Normal Human Monocytes. / Natarajan, Mohan; Vilayalaxmi; Szilagyl, Maria; Roldan, Francis N.; Meltz, Martin L.

In: Bioelectromagnetics, Vol. 23, No. 4, 05.2002, p. 271-277.

Research output: Contribution to journalArticle

Natarajan, Mohan ; Vilayalaxmi ; Szilagyl, Maria ; Roldan, Francis N. ; Meltz, Martin L. / NF-κB DNA-Binding Activity after High Peak Power Pulsed Microwave (8.2 GHz) Exposure of Normal Human Monocytes. In: Bioelectromagnetics. 2002 ; Vol. 23, No. 4. pp. 271-277.
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