Next-Generation AUC Adds a Spectral Dimension: Development of Multiwavelength Detectors for the Analytical Ultracentrifuge

Joseph Z. Pearson, Frank Krause, Dirk Haffke, Borries Demeler, Kristian Schilling, Helmut Cölfen

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

We describe important advances in analytical ultracentrifugation (AUC) hardware, which add new information to the hydrodynamic information observed in traditional AUC instruments. In contrast to the Beckman-Coulter XLA UV/visible detector, multiwavelength (MWL) detection is able to collect sedimentation data not just for one wavelength, but for a large wavelength range in a single experiment. The additional dimension increases the data density by orders of magnitude, significantly improving the statistics of the measurement and adding important information to the experiment since an additional dimension of spectral characterization is now available to complement the hydrodynamic information. The new detector avoids tedious repeats of experiments at different wavelengths and opens up new avenues for the solution-based investigation of complex mixtures. In this chapter, we describe the capabilities, characteristics, and applications of the new detector design with biopolymers as the focus of study. We show data from two different MWL detectors and discuss strengths and weaknesses of differences in the hardware and different data acquisition modes. Also, difficulties with fiber optic applications in the UV are discussed. Data quality is compared across platforms.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages1-26
Number of pages26
Volume562
DOIs
StatePublished - 2015

Publication series

NameMethods in Enzymology
Volume562
ISSN (Print)00766879
ISSN (Electronic)15577988

Keywords

  • Analytical ultracentrifugation
  • Hydrodynamic analysis
  • Multiwavelength detector
  • Optical detector development
  • Spectral analysis
  • UV-vis absorbance

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Pearson, J. Z., Krause, F., Haffke, D., Demeler, B., Schilling, K., & Cölfen, H. (2015). Next-Generation AUC Adds a Spectral Dimension: Development of Multiwavelength Detectors for the Analytical Ultracentrifuge. In Methods in Enzymology (Vol. 562, pp. 1-26). (Methods in Enzymology; Vol. 562). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2015.06.033