Neutrophil mediated tumor cell cytotoxicity: role of the peroxidase system

R. A. Clark, S. J. Klebanoff

Research output: Contribution to journalArticlepeer-review

226 Scopus citations

Abstract

A cytotoxic effect of human neutrophils on mammalian tumor cells is demonstrated. Cytotoxicity depends on the presence of intact neutrophils, phagocytosable particles, and a halide cofactor and is inhibited by azide, cyanide, and catalase. Neutrophils from patients with myeloperoxidase (MPO) deficiency or defective H2O2 production are not cytotoxic, but activity is restored by addition of purified MPO or H2O2 respectively. The findings support a mechanism involving the phagocytosis induced extracellular release of MPO and H2O2 and their reaction with a halide cofactor to damage the target cells.

Original languageEnglish (US)
Pages (from-to)1442-1447
Number of pages6
JournalJournal of Experimental Medicine
Volume141
Issue number6
DOIs
StatePublished - 1975
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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