Abstract
The kinetics of human blood-brain barrier neutral amino acid transport sites are described using isolated human brain capillaries as an in vitro model of the human blood-brain bazrrier. Kinetic parameters of transport (K(m), V(max), and K(D)) were determined for eight large neutral amino acids. K(m) values ranged from 0.30 ± 0.08 μM for phenylalanine to 8.8 ± 4.6 μM for valine. The amino acid analogs N-methylaminoisobutyric acid and 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid were used as model substrates of the alanine- and leucine-preferring transport systems, respectively. Phenylalanine is transported solely by the L-system (which is sensitive to 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid), and leucine is transported equally by the L- and ASC-system (which is sodium-dependent and N-methylaminoisobutyric acid-independent). Dose-dependent inhibition of the high affinity transport system by p-chloromercuribenzenesulfonic acid is demonstrated for phenylalanine, similar to the known sensitivity of blood-brain barrier transport in vivo. The K(m) values for the human brain capillary in vitro correlate significantly (r = 0.83, p < 0.01) with the K(m) values for the rat brain capillary in vivo. The results show that the affinity of human blood-brain barrier neutral amino acid transport is very high, i.e. very low K(m) compared to plasma amino acid concentrations. This provides a physical basis for the selective vulnerability of the human brain to derangements in amino acid availability caused by a selective hyperaminoacidemia, e.g. hyperphenylalaninemia.
Original language | English (US) |
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Pages (from-to) | 19392-19397 |
Number of pages | 6 |
Journal | Journal of Biological Chemistry |
Volume | 263 |
Issue number | 36 |
State | Published - 1988 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology