Neuroprotective effects of Toki-Shakuyaku-San (TJ-23) on glutamate induced neuronal death in cultured cerebellar granule cells

Xiang Qian Zhang, Nobuyoshi Hagino, Takehiro Nozaki

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The neuroprotective properties of Toki-Shakuyaku-San (TJ-23) were investigated in L-glutamic acid (Glu)induced neuronal death in 8 day primary cultured cerebellar granule cells. After a 4 h incubation in Mg2+-free Locke's solution at 37°C, the neuronal survival was 88.5%. An application of 100 μM of Glu into Mg2+-free Locke's solution resulted in 40% neuronal survival. Further application of either 0.05 or 0.5 mg/mL of TJ-23 into the above solution resulted in 65% and 71% of neuronal survival, respectively. In order to investigate the involvement of ions in the active mechanism of TJ-23, we examined Glu-induced neuronal death in the presence or absence of NaCl, Ca2+, and Mg2+. It was observed that TJ-23 selectively inhibited the Ca2+-dependent neuronal death. From these observations, it is inferred that TJ-23 has a neuroprotective effect on Glu-induced neuronal death.

Original languageEnglish (US)
Pages (from-to)107-112
Number of pages6
JournalPhytotherapy Research
Volume11
Issue number2
DOIs
StatePublished - Mar 1997
Externally publishedYes

Keywords

  • Cultured cerebellar granule cells
  • Glutamate neurotoxicity
  • Neuronal survival
  • Toki-Shakuyaku-San (TJ-23)

ASJC Scopus subject areas

  • Pharmacology

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