Neuropeptide specificity and inhibition of recombinant isoforms of the endopeptidase 3.4.24.16 family: Comparison with the related recombinant endopeptidase 3.4.24.15

Vanessa Rioli, Akira Kato, Fernanda C.V. Portaro, Gabriela K. Cury, Kai Te Kaat, Bruno Vincent, Frederic Checler, Antonio C.M. Camargo, Marc J. Glucksman, James L. Roberts, Shigehisa Hirose, Emer S. Ferro

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Endopeptidase EC 3.4.24.16 (EP24.16c, neurolysin) and thimet oligopeptidase EC 3.4.24.15 are close related members of a large family of metalloproteases. Besides their cytosolic and membrane bound form, endopeptidase EC 3.4.24.16 appears to be present in the inner membrane of the mitochondria (EP24.16m). We have overexpressed two porcine EP24.16 isoforms in E. coli and purified the recombinant proteins to homogeneity. We show here that these peptidases hydrolyse a series of neuropeptides with similar rates and at sites reminiscent of those elicited by classically purified human brain EP24.16c. All neuropeptides, except neurotensin, were similarly cleaved by recombinant endopeptidase 3.4.24.15 (EP24.15, thimet oligopeptidase), another zinc-containing metalloenzyme structurally related to EP24.16. These two EP24.16 isoforms were drastically inhibited by Pro-Ile and dithiothreitol and remained unaffected by a specific carboalkyl inhibitor (CFP-AAY-pAb) directed toward the related EP24.15. The present purification procedure of EP24.16 should allow to establish, by mutagenesis analysis, the mechanistic properties of the enzyme.

Original languageEnglish (US)
Pages (from-to)5-11
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume250
Issue number1
DOIs
StatePublished - Sep 8 1998
Externally publishedYes

Keywords

  • Endopeptidase
  • Peptide processing
  • Recombinant enzyme
  • pGEX

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Rioli, V., Kato, A., Portaro, F. C. V., Cury, G. K., Kaat, K. T., Vincent, B., Checler, F., Camargo, A. C. M., Glucksman, M. J., Roberts, J. L., Hirose, S., & Ferro, E. S. (1998). Neuropeptide specificity and inhibition of recombinant isoforms of the endopeptidase 3.4.24.16 family: Comparison with the related recombinant endopeptidase 3.4.24.15. Biochemical and Biophysical Research Communications, 250(1), 5-11. https://doi.org/10.1006/bbrc.1998.8941