Neuronal nitric oxide synthase control mechanisms in the cutaneous vasculature of humans in vivo

Dean L Kellogg Jr, Joan L. Zhao, Yubo Wu

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The physiological roles of constituitively expressed nitric oxide synthase (NOS) isoforms in humans, in vivo, are unknown. Cutaneous vasodilatation during both central nervous system-mediated, thermoregulatory reflex responses to whole-body heat stress and during peripheral axon reflex-mediated, local responses to skin warming in humans depend on nitric oxide (NO) generation by constituitively expressed NOS of uncertain isoform. We hypothesized that neuronal NOS (nNOS, NOS I) effects cutaneous vasodilatation during whole-body heat stress, but not during local skin warming. We examined the effects of the nNOS inhibitor 7-nitroindazole (7-NI) administered by intradermal microdialysis on vasodilatation induced by whole-body heat stress or local skin warming. Skin blood flow (SkBF) was monitored by laser-Doppler flowmetry (LDF). Blood pressure (MAP) was monitored and cutaneous vascular conductance calculated (CVC = LDF/MAP). In protocol 1, whole-body heat stress was induced with water-perfused suits. In protocol 2, local skin warming was induced through local warming units at LDF sites. At the end of each protocol, 56 mM sodium nitroprusside was perfused at microdialysis sites to raise SkBF to maximal levels for data normalization. 7-NI significantly attenuated CVC increases during whole-body heat stress (P < 0.05), but had no effect on CVC increases induced by local skin warming (P > 0.05). These diametrically opposite effects of 7-NI on two NO-dependent processes verify selective nNOS antagonism, thus proving that the nNOS isoform affects NO increases and hence vasodilatation during centrally mediated, reflex responses to whole-body heat stress, but not during locally mediated, axon reflex responses to local skin warming. We conclude that the constituitively expressed nNOS isoform has distinct physiological roles in cardiovascular control mechanisms in humans, in vivo.

Original languageEnglish (US)
Pages (from-to)847-857
Number of pages11
JournalJournal of Physiology
Volume586
Issue number3
DOIs
StatePublished - Feb 1 2008

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Nitric Oxide Synthase Type I
Skin
Hot Temperature
Vasodilation
Laser-Doppler Flowmetry
Reflex
Protein Isoforms
Nitric Oxide Synthase
Nitric Oxide
Microdialysis
Axons
Glycogen Synthase
Nitroprusside
Blood Vessels
Central Nervous System
Blood Pressure

ASJC Scopus subject areas

  • Physiology

Cite this

Neuronal nitric oxide synthase control mechanisms in the cutaneous vasculature of humans in vivo. / Kellogg Jr, Dean L; Zhao, Joan L.; Wu, Yubo.

In: Journal of Physiology, Vol. 586, No. 3, 01.02.2008, p. 847-857.

Research output: Contribution to journalArticle

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