Neurexin IV and Wrapper interactions mediate Drosophila midline glial migration and axonal ensheathment

Scott R. Wheeler, Swati Banerjee, Kevin Blauth, Stephen L. Rogers, Manzoor Bhat, Stephen T. Crews

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Glia play crucial roles in ensheathing axons, a process that requires an intricate series of glia-neuron interactions. The membrane-anchored protein Wrapper is present in Drosophila midline glia and is required for ensheathment of commissural axons. By contrast, Neurexin IV is present on the membranes of neurons and commissural axons, and is highly concentrated at their interfaces with midline glia. Analysis of Neurexin IV and wrapper mutant embryos revealed identical defects in glial migration, ensheathment and glial subdivision of the commissures. Mutant and misexpression experiments indicated that Neurexin IV membrane localization is dependent on interactions with Wrapper. Cell culture aggregation assays and biochemical experiments demonstrated the ability of Neurexin IV to promote cell adhesion by binding to Wrapper. These results show that neuronal-expressed Neurexin IV and midline glial-expressed Wrapper act as heterophilic adhesion molecules that mediate multiple cellular events involved in glia-neuron interactions.

Original languageEnglish (US)
Pages (from-to)1147-1157
Number of pages11
JournalDevelopment
Volume136
Issue number7
DOIs
StatePublished - Apr 1 2009
Externally publishedYes

Fingerprint

Neuroglia
Drosophila
Axons
Neurons
Cell Aggregation
Membranes
Cell Adhesion
Membrane Proteins
Embryonic Structures
Cell Culture Techniques

Keywords

  • Axon
  • Cell adhesion
  • Drosophila
  • Midline glia
  • Neurexin IV
  • Wrapper

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Neurexin IV and Wrapper interactions mediate Drosophila midline glial migration and axonal ensheathment. / Wheeler, Scott R.; Banerjee, Swati; Blauth, Kevin; Rogers, Stephen L.; Bhat, Manzoor; Crews, Stephen T.

In: Development, Vol. 136, No. 7, 01.04.2009, p. 1147-1157.

Research output: Contribution to journalArticle

Wheeler, Scott R. ; Banerjee, Swati ; Blauth, Kevin ; Rogers, Stephen L. ; Bhat, Manzoor ; Crews, Stephen T. / Neurexin IV and Wrapper interactions mediate Drosophila midline glial migration and axonal ensheathment. In: Development. 2009 ; Vol. 136, No. 7. pp. 1147-1157.
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