Nerve growth factor treatment enhances release of immunoreactive calcitonin gene-related peptide but not substance P from spinal dorsal horn slices in rats

Walter R. Bowles, Ma'Lou Sabino, Catherine Harding-Rose, Kenneth M. Hargreaves

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The effect of systemic nerve growth factor (NGF) on neuropeptide content and capsaicin-evoked release of neuropeptide from in vitro spinal cord dorsal horn slices was examined. Rats were injected subcutaneously every other day with murine NGF (mNGF) 1 mg/kg or saline for 7 days, or mNGF 0.1/kg, mNGF 1 mg/kg or saline for 13 days. Lumbar dorsal horn slices of the rat spinal cord from all groups showed a significant increase in immunoreactive calcitonin gene-related peptide (CGRP) release upon exposure to capsaicin. This release was enhanced in rats pretreated with mNGF 1 mg/kg for 7 days, but not after 13 days. No enhancement was seen after 7 or 13 days in any treatment group for immunoreactive substance P release. Upon examination of neuropeptide content in dorsal horn, no significant differences were noted between treatment groups. The increased iCGRP release from dorsal horn slices suggests a preferential release of CGRP and provides further evidence that NGF indirectly plays a role in the modulation of inflammation through the regulation of neuropeptide release.

Original languageEnglish (US)
Pages (from-to)239-242
Number of pages4
JournalNeuroscience Letters
Volume363
Issue number3
DOIs
StatePublished - Jun 17 2004

    Fingerprint

Keywords

  • Capsaicin
  • Dorsal spinal cord
  • Nerve growth factor
  • Neuropeptide
  • Nociceptor
  • Superfusion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this