Nerve growth factor-regulated properties of sensory neurones in Oct-2 null mutant mice

Natalia N. Ninkina, Vladimir L. Buchman, Armen N. Akopian, Sally N. Lawson, Miyuki Yamamoto, Elizabeth Campbell, Lynn Corcoran, John N. Wood

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The POU-domain transcription factor Oct-2 is expressed in both B lymphocytes and sensory neurones, where its expression is regulated by nerve growth factor (NGF). In order to define a possible role for Oct-2 in neurotrophin signalling, we examined the expression of an NGF-regulated channel (capsaicin-evoked ion fluxes), neuropeptides (substance P, calcitonin gene-related peptide), structural proteins (neurofilaments and peripherin) and receptors (trks) in dorsal root ganglion neurones derived from perinatal transgenic mice containing a defective Oct-2 structural gene. Northern blots show that central nervous tissue contains a larger than normal (> 10 kb) mRNA transcript corresponding in size to an Oct-2 transcript encoding a defective protein. PCR analysis shows the absence of normal Oct-2 transcripts in dorsal root ganglia. In null mutants, capsaisin sensitivity, and neuropeptide and cytoskeletal protein expression were unaffected by the loss of Oct-2 expression. The number of sensory neurones and the gross morphology of CNS tissues that normally express high levels of Oct-2 were also examined and found to be normal in the null mutant. Heterozygous animals show normal thresholds of sensitivity to noxious heat and normal inflammatory responses. Oct-2 does not therefore play an essential role in the NGF responsiveness of sensory neurones in these animals.

Original languageEnglish (US)
Pages (from-to)233-244
Number of pages12
JournalMolecular Brain Research
Volume33
Issue number2
DOIs
StatePublished - Nov 1995
Externally publishedYes

Keywords

  • Nerve growth factor
  • Null mutant
  • Oct-2
  • Sensory neuron

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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