Neoplastic conversion of human colon smooth muscle cells: No requirement for telomerase

Sitai Liang, Morton S. Kahlenberg, Dennis L. Rousseau, Peter J Hornsby

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The role of telomerase as an essential requirement for the neoplastic conversion of human cells has been controversial. In the model of conversion of normal human cells to cancer cells by the combination of simian virus 40 (SV40) early region genes and oncogenic Ras (H-RasG12V), telomerase (hTERT) was originally described as essential in conjunction with these other genes. Here we used primary cultures of colon smooth muscle cells isolated from surgical specimens. SV40 large T antigen (TAg) and oncogenic RasG12V were introduced into the cells by retroviral transduction and cells were rapidly transplanted into the subrenal capsule space in immunodeficient mice, without selection in culture. Malignant tumors were formed from transduced cells. Extensive invasion into the kidney occurred even when tumors were small; in contrast, at the same tumor size, oncogene-expressing fibroblasts did not show much invasion. Increased invasiveness was also observed in vitro. However, cells in these cancers showed morphological evidence of crisis, consistent with their lack of telomerase. These experiments on human colon smooth muscle cells support the concept that RasG12V and SV40 TAg form a minimal set of genes that can convert normal human cells to cancer cells without a requirement for hTERT.

Original languageEnglish (US)
Pages (from-to)478-484
Number of pages7
JournalMolecular Carcinogenesis
Volume47
Issue number6
DOIs
StatePublished - Jun 2008

Fingerprint

Telomerase
Smooth Muscle Myocytes
Colon
Simian virus 40
Neoplasms
Viral Tumor Antigens
ras Genes
Oncogenes
Genes
Capsules
Fibroblasts
Kidney

Keywords

  • Crisis
  • Immunodeficient mice
  • Invasion
  • Ras
  • SV40

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Neoplastic conversion of human colon smooth muscle cells : No requirement for telomerase. / Liang, Sitai; Kahlenberg, Morton S.; Rousseau, Dennis L.; Hornsby, Peter J.

In: Molecular Carcinogenesis, Vol. 47, No. 6, 06.2008, p. 478-484.

Research output: Contribution to journalArticle

Liang, Sitai ; Kahlenberg, Morton S. ; Rousseau, Dennis L. ; Hornsby, Peter J. / Neoplastic conversion of human colon smooth muscle cells : No requirement for telomerase. In: Molecular Carcinogenesis. 2008 ; Vol. 47, No. 6. pp. 478-484.
@article{e34e035112924497bb1a6add12376df1,
title = "Neoplastic conversion of human colon smooth muscle cells: No requirement for telomerase",
abstract = "The role of telomerase as an essential requirement for the neoplastic conversion of human cells has been controversial. In the model of conversion of normal human cells to cancer cells by the combination of simian virus 40 (SV40) early region genes and oncogenic Ras (H-RasG12V), telomerase (hTERT) was originally described as essential in conjunction with these other genes. Here we used primary cultures of colon smooth muscle cells isolated from surgical specimens. SV40 large T antigen (TAg) and oncogenic RasG12V were introduced into the cells by retroviral transduction and cells were rapidly transplanted into the subrenal capsule space in immunodeficient mice, without selection in culture. Malignant tumors were formed from transduced cells. Extensive invasion into the kidney occurred even when tumors were small; in contrast, at the same tumor size, oncogene-expressing fibroblasts did not show much invasion. Increased invasiveness was also observed in vitro. However, cells in these cancers showed morphological evidence of crisis, consistent with their lack of telomerase. These experiments on human colon smooth muscle cells support the concept that RasG12V and SV40 TAg form a minimal set of genes that can convert normal human cells to cancer cells without a requirement for hTERT.",
keywords = "Crisis, Immunodeficient mice, Invasion, Ras, SV40",
author = "Sitai Liang and Kahlenberg, {Morton S.} and Rousseau, {Dennis L.} and Hornsby, {Peter J}",
year = "2008",
month = "6",
doi = "10.1002/mc.20405",
language = "English (US)",
volume = "47",
pages = "478--484",
journal = "Molecular Carcinogenesis",
issn = "0899-1987",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Neoplastic conversion of human colon smooth muscle cells

T2 - No requirement for telomerase

AU - Liang, Sitai

AU - Kahlenberg, Morton S.

AU - Rousseau, Dennis L.

AU - Hornsby, Peter J

PY - 2008/6

Y1 - 2008/6

N2 - The role of telomerase as an essential requirement for the neoplastic conversion of human cells has been controversial. In the model of conversion of normal human cells to cancer cells by the combination of simian virus 40 (SV40) early region genes and oncogenic Ras (H-RasG12V), telomerase (hTERT) was originally described as essential in conjunction with these other genes. Here we used primary cultures of colon smooth muscle cells isolated from surgical specimens. SV40 large T antigen (TAg) and oncogenic RasG12V were introduced into the cells by retroviral transduction and cells were rapidly transplanted into the subrenal capsule space in immunodeficient mice, without selection in culture. Malignant tumors were formed from transduced cells. Extensive invasion into the kidney occurred even when tumors were small; in contrast, at the same tumor size, oncogene-expressing fibroblasts did not show much invasion. Increased invasiveness was also observed in vitro. However, cells in these cancers showed morphological evidence of crisis, consistent with their lack of telomerase. These experiments on human colon smooth muscle cells support the concept that RasG12V and SV40 TAg form a minimal set of genes that can convert normal human cells to cancer cells without a requirement for hTERT.

AB - The role of telomerase as an essential requirement for the neoplastic conversion of human cells has been controversial. In the model of conversion of normal human cells to cancer cells by the combination of simian virus 40 (SV40) early region genes and oncogenic Ras (H-RasG12V), telomerase (hTERT) was originally described as essential in conjunction with these other genes. Here we used primary cultures of colon smooth muscle cells isolated from surgical specimens. SV40 large T antigen (TAg) and oncogenic RasG12V were introduced into the cells by retroviral transduction and cells were rapidly transplanted into the subrenal capsule space in immunodeficient mice, without selection in culture. Malignant tumors were formed from transduced cells. Extensive invasion into the kidney occurred even when tumors were small; in contrast, at the same tumor size, oncogene-expressing fibroblasts did not show much invasion. Increased invasiveness was also observed in vitro. However, cells in these cancers showed morphological evidence of crisis, consistent with their lack of telomerase. These experiments on human colon smooth muscle cells support the concept that RasG12V and SV40 TAg form a minimal set of genes that can convert normal human cells to cancer cells without a requirement for hTERT.

KW - Crisis

KW - Immunodeficient mice

KW - Invasion

KW - Ras

KW - SV40

UR - http://www.scopus.com/inward/record.url?scp=44949242580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44949242580&partnerID=8YFLogxK

U2 - 10.1002/mc.20405

DO - 10.1002/mc.20405

M3 - Article

C2 - 18085530

AN - SCOPUS:44949242580

VL - 47

SP - 478

EP - 484

JO - Molecular Carcinogenesis

JF - Molecular Carcinogenesis

SN - 0899-1987

IS - 6

ER -