Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding

Alfred Nordheim, Eileen M Lafer, Lawrence J. Peck, James C. Wang, B. David Stollar, Alexander Rich

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

Negative superhelical coiling of covalently closed DNA plasmids facilitates the formation of lefthanded Z-DNA segments. This was demonstrated by binding of antibodies specific for Z-DNA. Plasmid pBR322 and two derivatives from it, pLP32 and pLP014, carrying inserts of alternating CG sequences of 32 bp and 14 bp, respectively, were used. Longer inserts required less negative superhelical density to induce the B-Z transitions. Antibody binding to supercoiled plasmids was also visualized by electron microscopy. Cross-linking of the antibody to the negatively supercoiled plasmid and restriction of the DNA with restriction endonucleases demonstrated that the antibodies combine with the CG insert in pLP32. For pBR322, however, evidence suggests that the antibody combines with a section of DNA containing 14 bases with alternating purine and pyrimidine residues with one residue out of alternation: CACGGGTGCGCATG. These crosslinking studies provide evidence for the binding specificity of the anti-Z-DNA antibodies. On the basis of experimental findings, we have calculated the changes in free energy associated with B-Z transitions in superhelical plasmids.

Original languageEnglish (US)
Pages (from-to)309-318
Number of pages10
JournalCell
Volume31
Issue number2 PART 1
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Z-Form DNA
Plasmids
Antibodies
DNA
Antinuclear Antibodies
DNA Restriction Enzymes
Electron Microscopy
Crosslinking
Electron microscopy
Free energy
Derivatives

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding. / Nordheim, Alfred; Lafer, Eileen M; Peck, Lawrence J.; Wang, James C.; David Stollar, B.; Rich, Alexander.

In: Cell, Vol. 31, No. 2 PART 1, 1982, p. 309-318.

Research output: Contribution to journalArticle

Nordheim, Alfred ; Lafer, Eileen M ; Peck, Lawrence J. ; Wang, James C. ; David Stollar, B. ; Rich, Alexander. / Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding. In: Cell. 1982 ; Vol. 31, No. 2 PART 1. pp. 309-318.
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