Negative elongation factor controls energy homeostasis in cardiomyocytes

Haihui Pan, Kunhua Qin, Zhanyong Guo, Yonggang Ma, Craig April, Xiaoli Gao, Thomas G. Andrews, Alex Bokov, Jianhua Zhang, Yidong Chen, Susan E Weintraub, Jian Bing Fan, Degeng Wang, Yanfen Hu, Gregory Aune, Merry L. Lindsey, Rong Li

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Negative elongation factor (NELF) is known to enforce promoter-proximal pausing of RNA polymerase II (PolII), a pervasive phenomenon observed across multicellular genomes. However, the physiological impact of NELF on tissue homeostasis remains unclear. Here, we show that whole-body conditional deletion of the B subunit of NELF (NELF-B) inadult mice results in cardiomyopathy and impaired response to cardiac stress. Tissue-specific knockout of NELF-B confirms its cell-autonomous function in cardiomyocytes. NELF directly supports transcription of those genes encoding rate-limiting enzymes in fatty acid oxidation (FAO) and the tricarboxylic acid (TCA) cycle. NELF also shares extensively transcriptional target genes with peroxisome proliferator-activated receptor α (PPARα), a master regulator of energy metabolism in the myocardium. Mechanistically, NELF helps stabilize the transcription initiation complex at the metabolism-related genes. Our findings strongly indicate that NELF is part of the PPARα-mediated transcription regulatory network that maintains metabolic homeostasis in cardiomyocytes.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalCell Reports
Volume7
Issue number1
DOIs
StatePublished - Oct 4 2014

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Cardiac Myocytes
Power control
Homeostasis
Transcription
Peroxisome Proliferator-Activated Receptors
Genes
Tissue homeostasis
negative elongation factor
Gene encoding
Citric Acid Cycle
RNA Polymerase II
Metabolic Networks and Pathways
Cardiomyopathies
Metabolism
Energy Metabolism
Myocardium
Fatty Acids
Genome
Tissue
Oxidation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Negative elongation factor controls energy homeostasis in cardiomyocytes. / Pan, Haihui; Qin, Kunhua; Guo, Zhanyong; Ma, Yonggang; April, Craig; Gao, Xiaoli; Andrews, Thomas G.; Bokov, Alex; Zhang, Jianhua; Chen, Yidong; Weintraub, Susan E; Fan, Jian Bing; Wang, Degeng; Hu, Yanfen; Aune, Gregory; Lindsey, Merry L.; Li, Rong.

In: Cell Reports, Vol. 7, No. 1, 04.10.2014, p. 79-85.

Research output: Contribution to journalArticle

Pan, H, Qin, K, Guo, Z, Ma, Y, April, C, Gao, X, Andrews, TG, Bokov, A, Zhang, J, Chen, Y, Weintraub, SE, Fan, JB, Wang, D, Hu, Y, Aune, G, Lindsey, ML & Li, R 2014, 'Negative elongation factor controls energy homeostasis in cardiomyocytes', Cell Reports, vol. 7, no. 1, pp. 79-85. https://doi.org/10.1016/j.celrep.2014.02.028
Pan, Haihui ; Qin, Kunhua ; Guo, Zhanyong ; Ma, Yonggang ; April, Craig ; Gao, Xiaoli ; Andrews, Thomas G. ; Bokov, Alex ; Zhang, Jianhua ; Chen, Yidong ; Weintraub, Susan E ; Fan, Jian Bing ; Wang, Degeng ; Hu, Yanfen ; Aune, Gregory ; Lindsey, Merry L. ; Li, Rong. / Negative elongation factor controls energy homeostasis in cardiomyocytes. In: Cell Reports. 2014 ; Vol. 7, No. 1. pp. 79-85.
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