Natural complex of cytokines is a potent stimulant to posttraumatic regeneration in rabbit cornea

L. V. Kovalchuk, I. P. Khoroshilova-Maslova, L. V. Gankovskaya, T. A. Krainova, R. A. Gundorova, L. V. Ilatovskaya, A. V. Marteushev, A. V. Tumanov

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


We elaborated an original technique based on local application of natural complex of cytokines (NCC) secreted by autologous peripheral leukocytes. In this study, we evaluated NCC influence on the healing of penetrating corneal wounds. NCC was derived from supernatants of PHA- stimulated rabbit peripheral leukocytes. Biological tests revealed file presence of IL-1, TNF, IL-6, MIF, and LIF in the complex. Chinchilla rabbits with standard penetrating corneal wounds received daily NCC instillation. The controls were instilled with cultural media 199 with antibiotics. At 24 hours, 3, 7, 14 and 30 days post-wounding, the rabbits were euthanized. We performed morphometry of corneal cross-sections stained with hematoxylin and eosin. In NCC-treated animals, we observed more vigorous migration and activation of neutrophils and macrophages followed by augmented resorption of fibrin. The later post-injury period (14-30 days) was marked with complete healing of the endothelial defect (in the controls, the mass of proliferating cells projected into the anterior eye chamber) and mature sear tissue with a higher content of fibrous component. NCC-treated eye scars were 1.6-fold thinner than the controls'. Local application of NCC promotes effective healing of posttraumatic cornea. It regulates all stages of regeneration and prevents rude scarring.

Original languageEnglish (US)
Pages (from-to)271-279
Number of pages9
JournalJournal of Ocular Pharmacology and Therapeutics
Issue number3
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Pharmacology
  • Pharmacology (medical)


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