Natural antioxidant dihydroxybenzyl alcohol blocks ritonavir-induced endothelial dysfunction in porcine pulmonary arteries and human endothelial cells

Sarah M. Weakley, Jun Jiang, Jianming Lü, Xinwen Wang, Peter H. Lin, Qizhi Yao, Changyi Chen

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Background: Patients with HIV have an increased incidence of pulmonary artery hypertension. This study was designed to determine if the naturally occurring antioxidant dihydroxybenzyl alcohol (DHBA) could counteract the deleterious effects of ritonavir (RTV), an HIV-protease inhibitor known to impair endothelial function and increase oxidative stress. Material/Methods: Antioxidant assays were performed on DHBA in a cell free system. Glutathione (GSH) levels were measured in human pulmonary artery endothelial cells (HPAEC) to determine the effect of DHBA on the level of oxidative stress in cells treated with RTV. Myograph analysis was performed on porcine pulmonary artery (PA) rings after treatment with RTV and/or DHBA. Likewise, reactive oxygen species (ROS) production was assessed in porcine PA rings after RTV +/- DHBA using a luci-genin reaction. Immunohistochemical staining for endothelial nitric oxide synthase (eNOS) was also performed in porcine PAs treated as above. Results: DHBA demonstrated significant antioxidant activity in a cell free system that surpassed that of vitamin C. Also, treatment with DHBA reduced RTV-induced reduction in endothelium-dependent vasorelaxation and eNOS staining and increased superoxide anion levels. Meanwhile, there was a reversal in RTV-induced oxidative stress leading to reduced GSH levels in HPAECs after treatment with DHBA. Conclusions: These findings suggest that the naturally occurring antioxidant DHBA reduces the impairment of vasomotor functions caused by RTV in porcine PAs and reduces oxidative stress caused by RTV in HPAEC and porcine PA rings. This study indicates that DHBA may have clinical applications in the prevention or treatment of antiretroviral drugs-associated vascular complications in patients with HIV.

Original languageEnglish (US)
Pages (from-to)BR235-BR241
JournalMedical Science Monitor
Volume17
Issue number9
DOIs
StatePublished - 2011
Externally publishedYes

Keywords

  • Antioxidant
  • Dihydroxybenzyl alcohol
  • Endothelial dysfunction
  • Endothelial nitric oxide synthase
  • Oxidative stress
  • Pulmonary artery hypertension
  • Ritonavir

ASJC Scopus subject areas

  • Medicine(all)

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