National epidemiology of mycoses survey: A multicenter study of strain variation and antifungal susceptibility among isolates of Candida species

M. A. Pfaller, S. A. Messer, A. Houston, M. S. Rangel-Frausto, T. Wiblin, H. M. Blumberg, J. E. Edwards, W. Jarvis, M. A. Martin, H. C. Neu, L. Saiman, Jan E Patterson, J. C. Dibb, C. M. Roldan, M. G. Rinaldi, R. P. Wenzel

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Abstract

The National Epidemiology of Mycoses Survey (NEMIS) involves six academic centers studying fungal infections in surgical and neonatal intensive care unit (ICU) patients. We studied variation in species and strain distribution and anti-fungal susceptibility of 408 isolates of Candida spp. Candida spp. were isolated from blood, other normally sterile site cultures, abscesses, wounds, catheters, and tissue biopsies of 141 patients hospitalized in the surgical (107 patients) and neonatal (34 patients) ICUs of medical centers located in Oregon, Iowa, California, Texas, Georgia and New York. Isolates were also obtained from selected colonized patients (16 patients) and the hands of health care workers (27 individuals). DNA typing was performed using pulsed field gel electrophoresis, and anti-fungal susceptibility to amphotericin B, 5-fluorocytosine, fluconazole, and itraconazole was determined using National Committee for Clinical Laboratory Standards (NCCLS) methods. Important variation in susceptibility to itraconazole and fluconazole was noted: MICs of itraconazole ranged from 0.25 μg/mL (MIC90) in Texas to 2.0 μg/mL (MIC90) in New York. Similarly, the MIC90 for fluconazole was higher for isolates from New York (64 μg/mL) compared to the other sites (8-16 μg/mL). In general, DNA typing revealed patient-unique strains; however, there were 13 instances of possible cross-infection noted in 5 of the medical centers. Notably, 9 of the 13 clusters involved species of Candida other than C. albicans. Potential transmission from patient-to-patient (C. Albicans, C. glabrata, C. tropicalis, C. parapsilosis) and health care worker-to-patient (C. albicans, C. parapsilosis, C. krusei) was noted in both surgical ICU and neonatal ICU settings. These data provide further insight into the epidemiology of nosocomial candidiasis in the ICU setting.

Original languageEnglish (US)
Pages (from-to)289-296
Number of pages8
JournalDiagnostic Microbiology and Infectious Disease
Volume31
Issue number1
DOIs
StatePublished - May 1998

Fingerprint

Mycoses
Candida
Multicenter Studies
Epidemiology
Itraconazole
Fluconazole
DNA Fingerprinting
Neonatal Intensive Care Units
Critical Care
Intensive Care Units
Surveys and Questionnaires
Delivery of Health Care
Flucytosine
Candidiasis
Pulsed Field Gel Electrophoresis
Amphotericin B
Cross Infection
Abscess
Catheters
Hand

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

National epidemiology of mycoses survey : A multicenter study of strain variation and antifungal susceptibility among isolates of Candida species. / Pfaller, M. A.; Messer, S. A.; Houston, A.; Rangel-Frausto, M. S.; Wiblin, T.; Blumberg, H. M.; Edwards, J. E.; Jarvis, W.; Martin, M. A.; Neu, H. C.; Saiman, L.; Patterson, Jan E; Dibb, J. C.; Roldan, C. M.; Rinaldi, M. G.; Wenzel, R. P.

In: Diagnostic Microbiology and Infectious Disease, Vol. 31, No. 1, 05.1998, p. 289-296.

Research output: Contribution to journalArticle

Pfaller, MA, Messer, SA, Houston, A, Rangel-Frausto, MS, Wiblin, T, Blumberg, HM, Edwards, JE, Jarvis, W, Martin, MA, Neu, HC, Saiman, L, Patterson, JE, Dibb, JC, Roldan, CM, Rinaldi, MG & Wenzel, RP 1998, 'National epidemiology of mycoses survey: A multicenter study of strain variation and antifungal susceptibility among isolates of Candida species', Diagnostic Microbiology and Infectious Disease, vol. 31, no. 1, pp. 289-296. https://doi.org/10.1016/S0732-8893(97)00245-9
Pfaller, M. A. ; Messer, S. A. ; Houston, A. ; Rangel-Frausto, M. S. ; Wiblin, T. ; Blumberg, H. M. ; Edwards, J. E. ; Jarvis, W. ; Martin, M. A. ; Neu, H. C. ; Saiman, L. ; Patterson, Jan E ; Dibb, J. C. ; Roldan, C. M. ; Rinaldi, M. G. ; Wenzel, R. P. / National epidemiology of mycoses survey : A multicenter study of strain variation and antifungal susceptibility among isolates of Candida species. In: Diagnostic Microbiology and Infectious Disease. 1998 ; Vol. 31, No. 1. pp. 289-296.
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