Nanovehicles as a novel target strategy for hyperthermic intraperitoneal chemotherapy: A multidisciplinary study of peritoneal carcinomatosis

Maciej Nowacki, Marek Wisniewski, Karolina Werengowska-Ciecwierz, Katarzyna Roszek, Joanna Czarnecka, I. Lakomska, Tomasz Kloskowski, Dominik Tyloch, Robert Debski, Katarzyna Pietkun, Marta Pokrywczynska, Dariusz Grzanka, Rafal Czajkowski, Gerard Drewa, A. Jundzill, Joseph K. Agyin, Samy L. Habib, Artur P. Terzyk, Tomasz Drewa

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In general, detection of peritoneal carcinomatosis (PC) occurs at the late stage when there is no treatment option. In the present study, we designed novel drug delivery systems that are functionalized with anti-CD133 antibodies. The C1, C2 and C3 complexes with cisplatin were introduced into nanotubes, either physically or chemically. The complexes were reacted with anti-CD133 antibody to form the labeled product of A0-o-CX-chem-CD133. Cytotoxicity screening of all the complexes was performed on CHO cells. Data showed that both C2 and C3 Pt-complexes are more cytotoxic than C1. Flow-cytometry analysis showed that nanotubes conjugated to CD133 antibody have the ability to target cells expressing the CD133 antigen which is responsible for the emergence of resistance to chemotherapy and disease recurrence. The shortest survival rate was observed in the control mice group (K3) where no hyperthermic intraperitoneal chemotherapy procedures were used. On the other hand, the longest median survival rate was observed in the group treated with A0-o-C1-chem-CD133. In summary, we designed a novel drug delivery system based on carbon nanotubes loaded with Pt-prodrugs and functionalized with anti-CD133 antibodies. Our data demonstrates the effectiveness of the new drug delivery system and provides a novel therapeutic modality in the treatment of melanoma.

Original languageEnglish (US)
Pages (from-to)22776-22798
Number of pages23
JournalOncotarget
Volume6
Issue number26
DOIs
StatePublished - Jan 1 2015

    Fingerprint

Keywords

  • Carcinomatosis
  • Hyperthermic intraperitoneal chemotherapy
  • Intraperitoneal perfusion and nanovehicles
  • Palliative

ASJC Scopus subject areas

  • Oncology

Cite this

Nowacki, M., Wisniewski, M., Werengowska-Ciecwierz, K., Roszek, K., Czarnecka, J., Lakomska, I., Kloskowski, T., Tyloch, D., Debski, R., Pietkun, K., Pokrywczynska, M., Grzanka, D., Czajkowski, R., Drewa, G., Jundzill, A., Agyin, J. K., Habib, S. L., Terzyk, A. P., & Drewa, T. (2015). Nanovehicles as a novel target strategy for hyperthermic intraperitoneal chemotherapy: A multidisciplinary study of peritoneal carcinomatosis. Oncotarget, 6(26), 22776-22798. https://doi.org/10.18632/oncotarget.4309