Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field

Saira F. Bakshi; Nataliia Guz; Andrey Zakharchenko; Han Deng; Alexei V. Tumanov; Craig D. Woodworth; Sergiy Minko; Dmitry M. Kolpashchikov; Evgeny Katz

doi:10.1039/c7nr08581h

Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field

Saira F. Bakshi
, Nataliia Guz
, Andrey Zakharchenko
, Han Deng
, Alexei V. Tumanov
, Craig D. Woodworth
, Sergiy Minko
, Dmitry M. Kolpashchikov
, Evgeny Katz

Department of Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A new biomimetic nanoreactor design, MaBiDz, is presented based on a copolymer brush in combination with superparamagnetic nanoparticles. This cellular nanoreactor features two species of magnetic particles, each functionalized with two components of a binary deoxyribozyme system. In the presence of a target mRNA analyte and a magnetic field, the nanoreactor is assembled to form a biocompartment enclosed by the polymeric brush that enables catalytic function of the binary deoxyribozyme with enhanced kinetics. MaBiDz was demonstrated here as a cellular sensor for rapid detection and imaging of a target mRNA biomarker for metastatic breast cancer, and its function shows potential to be expanded as a biomimetic organelle that can downregulate the activity of a target mRNA biomarker.

Original language	English (US)
Pages (from-to)	1356-1365
Number of pages	10
Journal	Nanoscale
Volume	10
Issue number	3
DOIs	https://doi.org/10.1039/c7nr08581h
State	Published - Jan 21 2018

ASJC Scopus subject areas

General Materials Science

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10.1039/c7nr08581h

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title = "Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field",

abstract = "A new biomimetic nanoreactor design, MaBiDz, is presented based on a copolymer brush in combination with superparamagnetic nanoparticles. This cellular nanoreactor features two species of magnetic particles, each functionalized with two components of a binary deoxyribozyme system. In the presence of a target mRNA analyte and a magnetic field, the nanoreactor is assembled to form a biocompartment enclosed by the polymeric brush that enables catalytic function of the binary deoxyribozyme with enhanced kinetics. MaBiDz was demonstrated here as a cellular sensor for rapid detection and imaging of a target mRNA biomarker for metastatic breast cancer, and its function shows potential to be expanded as a biomimetic organelle that can downregulate the activity of a target mRNA biomarker.",

author = "Bakshi, \{Saira F.\} and Nataliia Guz and Andrey Zakharchenko and Han Deng and Tumanov, \{Alexei V.\} and Woodworth, \{Craig D.\} and Sergiy Minko and Kolpashchikov, \{Dmitry M.\} and Evgeny Katz",

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doi = "10.1039/c7nr08581h",

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volume = "10",

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AU - Bakshi, Saira F.

AU - Guz, Nataliia

AU - Zakharchenko, Andrey

AU - Deng, Han

AU - Tumanov, Alexei V.

AU - Woodworth, Craig D.

AU - Minko, Sergiy

AU - Kolpashchikov, Dmitry M.

AU - Katz, Evgeny

PY - 2018/1/21

Y1 - 2018/1/21

N2 - A new biomimetic nanoreactor design, MaBiDz, is presented based on a copolymer brush in combination with superparamagnetic nanoparticles. This cellular nanoreactor features two species of magnetic particles, each functionalized with two components of a binary deoxyribozyme system. In the presence of a target mRNA analyte and a magnetic field, the nanoreactor is assembled to form a biocompartment enclosed by the polymeric brush that enables catalytic function of the binary deoxyribozyme with enhanced kinetics. MaBiDz was demonstrated here as a cellular sensor for rapid detection and imaging of a target mRNA biomarker for metastatic breast cancer, and its function shows potential to be expanded as a biomimetic organelle that can downregulate the activity of a target mRNA biomarker.

AB - A new biomimetic nanoreactor design, MaBiDz, is presented based on a copolymer brush in combination with superparamagnetic nanoparticles. This cellular nanoreactor features two species of magnetic particles, each functionalized with two components of a binary deoxyribozyme system. In the presence of a target mRNA analyte and a magnetic field, the nanoreactor is assembled to form a biocompartment enclosed by the polymeric brush that enables catalytic function of the binary deoxyribozyme with enhanced kinetics. MaBiDz was demonstrated here as a cellular sensor for rapid detection and imaging of a target mRNA biomarker for metastatic breast cancer, and its function shows potential to be expanded as a biomimetic organelle that can downregulate the activity of a target mRNA biomarker.

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M3 - Article

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AN - SCOPUS:85040907071

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SP - 1356

EP - 1365

JO - Nanoscale

JF - Nanoscale

IS - 3

ER -

Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field

Abstract

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