Nanomechanical biomarkers of single circulating tumor cells for detection of castration resistant prostate cancer

Pawel Osmulski; Devalingam Mahalingam; Maria E. Gaczynska; Joseph Liu; Susan Huang; Aaron M. Horning; Chiou Miin Wang; Ian M Thompson; Tim H.M. Huang; Chun Liang Chen

doi:10.1002/pros.22846

Nanomechanical biomarkers of single circulating tumor cells for detection of castration resistant prostate cancer

Pawel Osmulski, Devalingam Mahalingam, Maria E. Gaczynska, Joseph Liu, Susan Huang, Aaron M. Horning, Chiou Miin Wang, Ian M Thompson, Tim H.M. Huang, Chun Liang Chen

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

BACKGROUND Emerging evidence shows that nanomechanical phenotypes of circulating tumor cells (CTC) could become potential biomarkers for metastatic castration resistant prostate cancer (mCRPC). METHODS To determine the nanomechanical phenotypes of CTCs we applied atomic force microscopy (AFM) employing the PeakForce quantitative nanomechanical (QNM) imaging. We assessed biophysical parameters (elasticity, deformation, and adhesion) of 130 CTCs isolated from blood samples from five castration sensitive (CS) and 12 castration resistant prostate cancer (CRPCa) patients. RESULTS We found that CTCs from CRPCa patients are three times softer, three times more deformable, and seven times more adhesive than counterparts from CSPCa patients. Both nonsupervised hierarchical clustering and principle component analysis show that three combined nanomechanical parameters could constitute a valuable set to distinguish between CSPCa and CRPCa. CONLUSIONS Our study indicates that nanomechanical phenotypes of CTCs may serve as novel and effective biomarkers for mCRPC. Prostate 74: 1297-1307, 2014.

Original language	English (US)
Pages (from-to)	1297-1307
Number of pages	11
Journal	Prostate
Volume	74
Issue number	13
DOIs	https://doi.org/10.1002/pros.22846
State	Published - Sep 2014

Keywords

adhesion
biophysical biomarkers
deformability
elasticity
single-cell analysis

ASJC Scopus subject areas

Oncology
Urology

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10.1002/pros.22846

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title = "Nanomechanical biomarkers of single circulating tumor cells for detection of castration resistant prostate cancer",

abstract = "BACKGROUND Emerging evidence shows that nanomechanical phenotypes of circulating tumor cells (CTC) could become potential biomarkers for metastatic castration resistant prostate cancer (mCRPC). METHODS To determine the nanomechanical phenotypes of CTCs we applied atomic force microscopy (AFM) employing the PeakForce quantitative nanomechanical (QNM) imaging. We assessed biophysical parameters (elasticity, deformation, and adhesion) of 130 CTCs isolated from blood samples from five castration sensitive (CS) and 12 castration resistant prostate cancer (CRPCa) patients. RESULTS We found that CTCs from CRPCa patients are three times softer, three times more deformable, and seven times more adhesive than counterparts from CSPCa patients. Both nonsupervised hierarchical clustering and principle component analysis show that three combined nanomechanical parameters could constitute a valuable set to distinguish between CSPCa and CRPCa. CONLUSIONS Our study indicates that nanomechanical phenotypes of CTCs may serve as novel and effective biomarkers for mCRPC. Prostate 74: 1297-1307, 2014.",

keywords = "adhesion, biophysical biomarkers, deformability, elasticity, single-cell analysis",

author = "Pawel Osmulski and Devalingam Mahalingam and Gaczynska, {Maria E.} and Joseph Liu and Susan Huang and Horning, {Aaron M.} and Wang, {Chiou Miin} and Thompson, {Ian M} and Huang, {Tim H.M.} and Chen, {Chun Liang}",

year = "2014",

month = sep,

doi = "10.1002/pros.22846",

language = "English (US)",

volume = "74",

pages = "1297--1307",

journal = "Prostate",

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publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Nanomechanical biomarkers of single circulating tumor cells for detection of castration resistant prostate cancer

AU - Osmulski, Pawel

AU - Mahalingam, Devalingam

AU - Gaczynska, Maria E.

AU - Liu, Joseph

AU - Huang, Susan

AU - Horning, Aaron M.

AU - Wang, Chiou Miin

AU - Thompson, Ian M

AU - Huang, Tim H.M.

AU - Chen, Chun Liang

PY - 2014/9

Y1 - 2014/9

N2 - BACKGROUND Emerging evidence shows that nanomechanical phenotypes of circulating tumor cells (CTC) could become potential biomarkers for metastatic castration resistant prostate cancer (mCRPC). METHODS To determine the nanomechanical phenotypes of CTCs we applied atomic force microscopy (AFM) employing the PeakForce quantitative nanomechanical (QNM) imaging. We assessed biophysical parameters (elasticity, deformation, and adhesion) of 130 CTCs isolated from blood samples from five castration sensitive (CS) and 12 castration resistant prostate cancer (CRPCa) patients. RESULTS We found that CTCs from CRPCa patients are three times softer, three times more deformable, and seven times more adhesive than counterparts from CSPCa patients. Both nonsupervised hierarchical clustering and principle component analysis show that three combined nanomechanical parameters could constitute a valuable set to distinguish between CSPCa and CRPCa. CONLUSIONS Our study indicates that nanomechanical phenotypes of CTCs may serve as novel and effective biomarkers for mCRPC. Prostate 74: 1297-1307, 2014.

AB - BACKGROUND Emerging evidence shows that nanomechanical phenotypes of circulating tumor cells (CTC) could become potential biomarkers for metastatic castration resistant prostate cancer (mCRPC). METHODS To determine the nanomechanical phenotypes of CTCs we applied atomic force microscopy (AFM) employing the PeakForce quantitative nanomechanical (QNM) imaging. We assessed biophysical parameters (elasticity, deformation, and adhesion) of 130 CTCs isolated from blood samples from five castration sensitive (CS) and 12 castration resistant prostate cancer (CRPCa) patients. RESULTS We found that CTCs from CRPCa patients are three times softer, three times more deformable, and seven times more adhesive than counterparts from CSPCa patients. Both nonsupervised hierarchical clustering and principle component analysis show that three combined nanomechanical parameters could constitute a valuable set to distinguish between CSPCa and CRPCa. CONLUSIONS Our study indicates that nanomechanical phenotypes of CTCs may serve as novel and effective biomarkers for mCRPC. Prostate 74: 1297-1307, 2014.

KW - adhesion

KW - biophysical biomarkers

KW - deformability

KW - elasticity

KW - single-cell analysis

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Nanomechanical biomarkers of single circulating tumor cells for detection of castration resistant prostate cancer

Abstract

Keywords

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