Nano-LISA for in vitro diagnostic applications

Saher Maswadi, Randolph D. Glickman, Rowe Elliot, Norman Barsalou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

We previously reported the detection of bacterial antigen with immunoaffinity reactions using laser optoacoustic spectroscopy and antibody-coupled gold nanorods (Ab-NR) as a contrast agent specifically targeted to the antigen of interest. The Nano-LISA (Nanoparticle Linked Immunosorbent Assay) method has been adapted to detect three very common blood-borne viral infectious agents, i.e. human T-lymphotropic virus (HTLV), human immunodeficiency virus (HIV) and hepatitis-B (Hep-B). These agents were used in a model test panel to illustrate the performance of the Nano-LISA technique. A working laboratory prototype of a Nano-LISA microplate reader-sensor was assembled and tested against the panel containing specific antigens of each of the infectious viral agents. Optoacoustic (OA) responses generated by the samples were detected using the probe beam deflection technique, an all-optical, non-contact technique. A LabView graphical user interface was developed for control of the instrument and real-time display of the test results. The detection limit of Nano-LISA is at least 1 ng/ml of viral antigen, and can reach 10 pg/ml, depending on the binding affinity of the specific detection antibody used to synthesize the Ab-NR. The method has sufficient specificity, i.e. the detection reagents do not cross-react with noncomplementary antigens. Thus, the OA microplate reader, incorporating NanoLISA, has adequate detection sensitivity and specificity for use in clinical in vitro diagnostic testing.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2011
DOIs
Publication statusPublished - May 5 2011
EventPhotons Plus Ultrasound: Imaging and Sensing 2011 - San Francisco, CA, United States
Duration: Jan 23 2011Jan 25 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7899
ISSN (Print)1605-7422

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2011
CountryUnited States
CitySan Francisco, CA
Period1/23/111/25/11

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Keywords

  • Gold
  • HIV
  • HLTV
  • Hep-B
  • In vitro diagnostic
  • NanoLISA
  • Nanoparticles
  • Optoacoustic spectroscopy
  • Pathogenic detection
  • Probe beam deflection technique
  • Ultrasound optical sensor

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Maswadi, S., Glickman, R. D., Elliot, R., & Barsalou, N. (2011). Nano-LISA for in vitro diagnostic applications. In Photons Plus Ultrasound: Imaging and Sensing 2011 [78991O] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7899). https://doi.org/10.1117/12.874922