NAD-dependent 11β-hydroxysteroid dehydrogenase in cultured human colonic epithelial cells

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10 Scopus citations


The inactivation of physiological glucocorticoids by 11β-hydroxysteroid dehydrogenase (11β-HSD) confers mineralocorticoid specificity to certain aldosterone target tissues. However, 11β-HSD activity in a human mineralocorticoid-responsive tissue has never been characterized. The present studies describe the features of 11β-HSD in the cultured human colonic epithelial cell line, T84. The 11β-HSD activity of T84 cells resided in the microsomal fraction and showed a marked preference for NAD rather than NADP as cofactor. NAD or NADP (200 μM) increased the conversion of corticosterone to 11-dehydrocorticosterone by 24.1 ± 2.1 and 0.5 ± 0.7 pmol · mg protein-1 · 20 min-1, respectively, indicating a > 40-fold preference for NAD vs. NADP. The Michaelis constant values for corticosterone and cortisol were 11.3 ± 1.5 and 79.8 ± 10 nM, respectively. The T84 11β-HSD was inhibited by 11-dehydrocorticosterone in a noncompetitive fashion [inhibition constant (K(i)) = 180 ± 9.6 nM] and by carbenoxolone in a competetive fashion (K(i) = 17.4 ± 1.3 nM). The expression of mineralocorticoid receptors in these cells was demonstrated by reverse transcriptase-polymerase chain reaction of mRNA isolated from T84 cells and by [3H] aldosterone binding studies. The coexpression of this NAD-dependent isoform of 11β-HSD and mineralocorticoid receptors is consistent with the view that the NAD-dependent isoform is responsible for the specificity of mineralocorticoid responses.

Original languageEnglish (US)
Pages (from-to)C1467-C1473
JournalAmerican Journal of Physiology - Cell Physiology
Issue number6 37-6
StatePublished - Jan 1 1995
Externally publishedYes


  • T84 cell
  • colon
  • glucocorticoids
  • mineralocorticoids

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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