N-Myc downstream-regulated gene 2 (NDRG2) inhibits glioblastoma cell proliferation

Yanchun Deng, Libo Yao, Ling Chau, Samuel Sai Ming Ng, Ying Peng, Xinping Liu, Wo Shing Au, Jicun Wang, Fuyang Li, Shaoping Ji, Hua Han, Xiaoyan Nie, Qing Li, Hsiang Fu Kung, Suet Yi Leung, Marie Chia Mi Lin

Research output: Contribution to journalArticle

172 Scopus citations

Abstract

The most severe form of brain glioma, glioblastoma (GBM), is highly malignant and usually resistant to chemotherapy. Therefore, discovery of new targets for gene therapy is important. Using subtraction cloning, we identified the human N-Myc downstream-regulated gene 2 (hNDRG2), located at chromosome 14q11.2, as a gene that is significantly suppressed in GBM tissues. Semiquantitative RT-PCR showed that the hNDRG2 gene transcript is expressed in normal brain tissue and low-grade gliomas but is present at low levels in 15 of 27 (56%) human GBM tissues and all of the 6 human glioblastoma cell lines examined. Furthermore, transfection of human glioblastoma U373 and U138 cells with a cDNA encoding hNDRG2 markedly reduced the cell proliferation. Our findings provide the first evidence to suggest that hNDRG2 may play a role in glioblastoma carcinogenesis.

Original languageEnglish (US)
Pages (from-to)342-347
Number of pages6
JournalInternational Journal of Cancer
Volume106
Issue number3
DOIs
StatePublished - Sep 1 2003

Keywords

  • Cell proliferation
  • Gene expression
  • Glioblastoma
  • HNDRG2
  • U373

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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