N-glycosylation is not essential for enzyme activity of 11β- hydroxysteroid dehydrogenase type 2

Zhetcho N. Kyossev, W. Brian Reeves

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


11β-hydroxysteroid dehydrogenase (11βHSD) catalyzes the oxidation of cortisol and corticosterone to cortisone and 11-dehydrocorticosterone, respectively. NAD-dependent 11β-HSD is expressed at high levels in the distal nephron and contributes to mineralocorticoid specificity in that region. The present studies determined whether N-glycosylation is necessary for the activity of NAD-dependent 11β-HSD (11β-HSD2). First, cultured human colonic epithelial cells (T84 cells), which express native 11β-HSD2 activity, were grown in medium with and without tunicamycin, an inhibitor of N-glycosylation. Tunicamycin had no effect on the enzyme activity. Next, the only putative N-glycosylation site (Asn394-Leu395-Ser396) of the cloned human kidney enzyme was eliminated by site-directed mutagenesis. Chinese hamster ovary (CHO) cells transfected with either the wild-type or the mutant cDNA construct showed no difference in the expressed enzyme activity, and Western blot analysis showed that the 11β-HSD2 protein was the same size in cells expressing either the wild-type or the N394D mutant. Likewise, the molecular mass of the 11β-HSD2 protein in T84 cells was not altered by treatment with peptide-N-glycosidase F or tunicamycin. We conclude that human 11β-HSD2 is not a N-glycoprotein and N-glycosylation is not essential for the expression of enzyme activity.

Original languageEnglish (US)
Pages (from-to)682-686
Number of pages5
JournalKidney international
Issue number3
StatePublished - 1997
Externally publishedYes


  • 11β-hydrozysteroid dehydrogenase
  • Aldosterone
  • Colon
  • Glycosylation
  • Kidney
  • Mutagenesis
  • N-glycosylation

ASJC Scopus subject areas

  • Nephrology


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