Myeloperoxidase-induced inactivation of α1-antiprotease in hamsters

M. C. Zaslow, Robert A Clark, P. J. Stone, J. Calore, G. L. Snider, C. Franzblau

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

While numerous studies have demonstrated that the myeloperoxidase system found in neutrophils can oxidize and functionally inactivate α1-proteinase inhibitor in vitro, there is little direct evidence that this phenomenon is relevant in vivo. Using incubation with tritiated porcine pancreatic elastase followed by column chromatography to quantitate binding, we demonstrated recovery of microgram amounts of functional α1-protease inhibitor from bronchoalveolar lavage of hamster lungs. When exposed to the myeloperoxidase system in vitro, hamster α1-protease inhibitor was 97% inactivated. Functional α1-protease inhibitor recovered by bronchoalveolar lavage 20 minutes after hamsters were given intratracheal injections with myeloperoxidase and either hydrogen peroxide or glucose plus glucose oxidase was only half that recovered from control animals. These studies suggest that the myeloperoxidase system is effective in oxidizing α1-protease inhibitor in vivo. They support the concept that oxidation of α1-protease inhibitor by myeloperoxidase from neutrophil granules in the presence of H2O2 and halide may produce elastase-antielastase imbalance in vivo and contribute to the development of acute lung injury and emphysema in humans.

Original languageEnglish (US)
Pages (from-to)178-184
Number of pages7
JournalJournal of Laboratory and Clinical Medicine
Volume105
Issue number2
StatePublished - 1985
Externally publishedYes

Fingerprint

Protease Inhibitors
Cricetinae
Peroxidase
Pancreatic Elastase
Bronchoalveolar Lavage
Neutrophils
Glucose Oxidase
Column chromatography
Acute Lung Injury
Emphysema
Hydrogen Peroxide
Chromatography
Animals
Peptide Hydrolases
Swine
Glucose
Recovery
Oxidation
Lung
Injections

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Zaslow, M. C., Clark, R. A., Stone, P. J., Calore, J., Snider, G. L., & Franzblau, C. (1985). Myeloperoxidase-induced inactivation of α1-antiprotease in hamsters. Journal of Laboratory and Clinical Medicine, 105(2), 178-184.

Myeloperoxidase-induced inactivation of α1-antiprotease in hamsters. / Zaslow, M. C.; Clark, Robert A; Stone, P. J.; Calore, J.; Snider, G. L.; Franzblau, C.

In: Journal of Laboratory and Clinical Medicine, Vol. 105, No. 2, 1985, p. 178-184.

Research output: Contribution to journalArticle

Zaslow, MC, Clark, RA, Stone, PJ, Calore, J, Snider, GL & Franzblau, C 1985, 'Myeloperoxidase-induced inactivation of α1-antiprotease in hamsters', Journal of Laboratory and Clinical Medicine, vol. 105, no. 2, pp. 178-184.
Zaslow, M. C. ; Clark, Robert A ; Stone, P. J. ; Calore, J. ; Snider, G. L. ; Franzblau, C. / Myeloperoxidase-induced inactivation of α1-antiprotease in hamsters. In: Journal of Laboratory and Clinical Medicine. 1985 ; Vol. 105, No. 2. pp. 178-184.
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