Myeloperoxidase H2O2 halide system

cytotoxic effect on human blood leukocytes

Robert A Clark, S. J. Klebanoff

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Myeloperoxidase, H2O2, and a halide form a potent antimicrobial and cytotoxic system of the polymorphonuclear leukocyte. A cytotoxic effect of this system on human blood leukocytes is demonstrated, employing 51Cr release and dye exclusion assays. Cytotoxicity is dependent on enzymatically active myeloperoxidase, H2O2, or a peroxide generating enzyme system and either chloride or iodide. Cell damage is rapid, with maximal levels of 51Cr release occurring within 30 to 60 min. Approximately equal sensitivity to the peroxidase system is observed for polymorphonuclear leukocytes and mononuclear leukocytes. Since myeloperoxidase and H2O2 are released from polymorphonuclear leukocytes under certain conditions, such as during particle ingestion, it is suggested that peroxidase mediated leukocyte injury may be an important feature of the inflammatory response.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalBlood
Volume50
Issue number1
StatePublished - 1977
Externally publishedYes

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Peroxidase
Leukocytes
Blood
Neutrophils
Mononuclear Leukocytes
Peroxides
Iodides
Cytotoxicity
Chlorides
Assays
Coloring Agents
Eating
Cells
Wounds and Injuries
Enzymes

ASJC Scopus subject areas

  • Hematology

Cite this

Myeloperoxidase H2O2 halide system : cytotoxic effect on human blood leukocytes. / Clark, Robert A; Klebanoff, S. J.

In: Blood, Vol. 50, No. 1, 1977, p. 65-70.

Research output: Contribution to journalArticle

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AB - Myeloperoxidase, H2O2, and a halide form a potent antimicrobial and cytotoxic system of the polymorphonuclear leukocyte. A cytotoxic effect of this system on human blood leukocytes is demonstrated, employing 51Cr release and dye exclusion assays. Cytotoxicity is dependent on enzymatically active myeloperoxidase, H2O2, or a peroxide generating enzyme system and either chloride or iodide. Cell damage is rapid, with maximal levels of 51Cr release occurring within 30 to 60 min. Approximately equal sensitivity to the peroxidase system is observed for polymorphonuclear leukocytes and mononuclear leukocytes. Since myeloperoxidase and H2O2 are released from polymorphonuclear leukocytes under certain conditions, such as during particle ingestion, it is suggested that peroxidase mediated leukocyte injury may be an important feature of the inflammatory response.

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